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Histology

What services do we offer?

  1. Embedding into Paraffin with the STP 420 D dehydration/infiltration unit (Microm) and the EGF 1160 embedding station (Leica). This includes stepwise dehydration in a graded alcohol series, transfer to xylol, as well as paraffin infiltration, sample orientation and embedding.

  2. Embedding into Technovit 7100 (normal tissues, embryos) or Technovit 9100 (tissues including bone or cartilage), normally by hand. We are currently trying to establish a rapid microwave-assisted and automated embedding protocol using the Leica AMW.

  3. Microtomy: We section your paraffin or Technovit embedded samples (Microm HM 340E, Microm HM 355S; section thickness 1-10 µm) as well as gelatin embedded and frozen samples (cryostat Microm HM 560, section thickness 3-20 µm).

  4. Histological stainings: Routinely we stain paraffin sections with hematoxylin-eosin (HE) or Giemsa, but upon request also other stainings such as  modified Goldner, Azan, acid fuchsin orange G (AFOG) or Mallory can be performed (Fig. 1). Technovit sections are stained with toluidine blue + borax (Fig. 2).
Figure 1: Stainings on paraffin sections. A Mouse intestine, mucosa, HE. B Human Skin, Goldner. C Zebrafish brain, AFOG. D Zebrafish brain, Mallory.

Figure 2: Toluidine blue staining of a frontal plastic section (Technovit 7100) through an axolotl larva. The left eye is shown at higher magnification.

Immunohistology of cryosections or paraffin sections: e.g. immunofluorescence, AEC-Romulin staining (Fig. 3A,B). Upon request we also offer whole mount immunofluorescence with subsequent embedding of stained tissues into Technovit 7100 (Fig. 3C,D).


Figure 3: Immunohistology. A S-100 in human skin, paraffin section stained with anti S-100 and HRP-DAB. B Sox-9 in pelletet chondrocytes, detection as in A. C?-integrin in the smooth muscle layers of Xenopus intestine. Wholemount staining followed by Technovit embedding and sectioning. D ?1-integrin (green) and ?-catenin (red) in Xenopus enterocytes, wholemount staining as in C. A capillary with red blood cells can be seen in the lamina propria.

General support:
If you have any specific requests for sample preparation and staining not covered by the list, feel free to ask! Maybe we can find a solution for your problem.

Good to know in addition

Semithin sections of epon-embedded tissues are a valuable alternative to paraffin or Technovit embedded specimens. Epon was designed for ultrathin sectioning and TEM-analysis. Thin sections have an improved resolution. In addition, use of strong EM-grade fixatives such as glutaraldehyde and osmium tetroxide as well as block staining with uranyl acetate result in excellent tissue preservation, which becomes apparent also at the light microscopical level (Fig. 4). Samples can be processed by hand, with the Leica tissue processor (EM-TP) or with a microwave-based tissue processor (Leica AMW). We can offer these options in collaboration with the EM-Facility of the CRTD.

Figure 4: Semithin Epon sections (1 µm) through the tail of an axolotl larva (A) and the eye of a zebrafish larva (B); my, myotome; not, notochord; nt, neural tube.

Who we are:

The Histology Facility is scientifically guided by PhD Thomas Kurth, who is an expert in Developmental Biology, Histology and Electron Microscopy. Dr. Denis Corbeil (Group Leader in the BIOTEC), is  scientifically advising the Histology Facility with his substancial  expertise in this field. Technical assistance and service is provided by Anja Ahrendt (BSc Molecular Biotechnology), Susanne Weiche (Biologist), Suzanne Manthey (MTA, Universitätsklinikum) and Annett Wenke (MTA, Universtätsklinikum).

Contact:


Anja Ahrendt, Tel. 49-351-463-40015, anja.ahrendt@biotec.tu-dresden.de

Thomas Kurth, Tel. 49-351-458-82090, thomas.kurth@crt-dresden.de

Susanne Weiche, Tel. 49-351-463-40015, susanne.weiche@biotec.tu-dresden.de


The Histology Facility is supported by EFRE.

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