FACS Facility
Services we offer:
The BIOTEC/CRTD-FACS Facility comprehends three state-of-the-art benchtop machines.
| Becton Dickinson FACS ARIA II SORP (cell sorter) - location: BIOTEC | Becton Dickinson LSR II (analyzer) location: BIOTEC |
|---|---|
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| Becton Dickinson FACS Calibur (analyzer) location: MTZ | Becton Dickinson LSR II (analyzer) location: MTZ |
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Equipment:
BD FACS Aria II (location BIOTEC):
- measurement of 16 parameters: 14 fluorescence detectors, FSC (forward scatter) and SSC (side scatter)
- 5 lasers: Red (640nm), YellowGreen (561nm), Blue (488nm), Violet (405nm), UV (355nm)
- 2 way sort into 15ml Falcon tubes, 2 & 4 way sort into FACS tubes and microtubes, slides and plates (6, 24, 48, 96 and 384-well)
- The cell sorter can only be used with service - please use this form and mail it the person in charge, Nicole Rund
(or phone 0351/46340119)
BD LSR II (location BIOTEC):
- measurement of 10 parameters: 8 fluorescence detectors, FSC and SSC
- 3 lasers: Red (633nm), Blue (488nm), Violet (405nm)
- you can measure out of tubes or plates (HTS-System)
- For using the LSRII you need an introduction. For introduction, please contact Nicole Rund. After being introduced you can use the online system to book the time for your analysis & to clean up. Booking system
BD LSR II (location MTZ):
- measurement of 15 parameters: 13 fluorescence detectors, FSC and SSC
- 4 lasers: Red (635nm), Blue (488nm), Violet (407nm), UV (355nm)
- For using the LSRII you need an introduction by BD. The classes are organized by Andrea Stephan at the Institute for Physiological Chemistry, please contact her directly for the next available spot in an introductory class. After being introduced please provide your certificate from BD to Silvia Palme - she will create your user account and you can then use the online system to book the time for your analysis & to clean up Booking system
BD FACS Calibur (location MTZ):
- measurement of 6 parameters: FSC (forward scatter) and SSC (side scatter), 4 fluorescence detectors
- 2 lasers: Red (635nm), Blue (488nm)
- For using the Calibur, you need an introduction. For introduction, please contact Nicole Rund.
Good to know in addition:
Frequently asked questions about cell sorting:
Basic information about FACS:
- Becton Dickinson: Training
- invitrogen: Tutorial
- UMASS: Mario Roederer Seminar & Fluorochrome Error Contribution
Spectra viewer:
Societies and Networks:
- PURDUE University Flow
- ISAC - International Society for Advancement of Cytometry
- Stanford Shared FACS Facility
- German Society for Cytometry
- DARTMOUTH Flow
- Schuitemaker lab
- WEHI Flow
- UMASS - University of Massachusetts Medical School
Literature:
- Howard Shapiro - Practical Flow Cytometry
(you can download from the website Beckman Coulter) - Mario Roederer - Compensation
- Alice Longobardi Givan - Flow cytometry - first principles (second edition)
- Michael G. Ormerod - Flow Cytometry, a basic introduction
- Michael G. Ormerod - Flow Cytometry (third edition)
Who we are:
The FACS Facility is scientifically guided by Dr. Denis Corbeil. Technical assistance and service is provided by Medical Technician, Nicole Rund, who has years of experience in working with FACS.
Contact:
For BD FACS Aria II and BD LSR II (location BIOTEC):
Nicole Rund, nicole.rund(at)biotec.tu-dresden.de
Phone: 0351-463 40119
BIOTEC, room 242
For BD FACS Calibur (location MTZ):
Nicole Rund, nicole.rund(at)biotec.tu-dresden.de
Phone: 0351-463 40119
For BD LSR II (location MTZ):
Silvia Palme, silvia.palme(at)crt-dresden.de
Phone: 0351-458 6454
Opening times:
Aria II (location BIOTEC): Monday to Friday - 9am to 5pm
LSR II (location BIOTEC): 24h
LSR II (location MTZ): Monday to Friday - 8am to 5pm
FACS Calibur (location MTZ): 24h
The BIOTEC/CRTD-FACS Facility is supported by EFRE.
