Examplepictures of DNA-Structures

Jörg Mansfeld – Proteolysis in Cell Cycle and Cell Fate Decisions

2004-2008: PhD in Biology, Institute of Biochemistry, ETH-Zurich, Zurich, Switzerland.

2008-2012: Postdoctoral fellow at the Gurdon Institute/Cancer Research UK, Cambridge University, Cambridge, UK.

 from 2013: Emmy Noether group leader, BIOTEC, TU Dresden, Germany.


The coordination of proliferation with quiescence and differentiation is fundamental for multicellular life and development. The decision on whether cells keep dividing, or whether they temporally or permanently exit from the cell cycle is tightly controlled by ubiquitin-mediated proteolysis. Ubiquitin-mediated proteolysis requires the sequential interplay of three enzymes E1, E2, and ubiquitin E3 ligases. E3 ligases recognise and covalently modify crucial substrates such cell cycle regulators with chains of ubiquitin molecules and thereby target them for degradation by the 26S proteasome. Emerging evidence indicates that mutations in components of the ubiquitin proteasome system are a hallmark of multiple cancers and in addition interfere with faithful development and differentiation.

Our lab combines cell biological and biochemical approaches to reveal how the ubiquitin system controls cell cycle progression and the exit into quiescence and differentiation using human cells a model. For more information on our research please visit our lab page.


Katharina B Beer, Gholamreza Fazeli, Kristyna Judasova, Linda Irmisch, Jona Causemann, Jörg Mansfeld, Ann M Wehman (2019). Degron-tagged reporters probe membrane topology and enable the specific labelling of membrane-wrapped structures. Nature Communications 10:3490

Anna K.L. Liess*, Alena Kucerova*, Kristian Schweimer, Lu Yu, Theodoros I. Roumeliotis, Mathias Diebold, Olexandr Dybkov, Christoph Sotriffer, Henning Urlaub, Jyoti S. Choudhary, Jörg Mansfeld#, Sonja Lorenz# (2019). Autoinhibition Mechanism of the Ubiquitin-Conjugating Enzyme UBE2S by Autoubiquitination. Structure. 2019 Jun 20,  doi: 10.1016/j.str.2019.05.008         *shared first authorship    #corresponding authors

Bakos, G., Yu l., Gak IA., Roumeliotis, TI., Liakopoulos D., Choudhary JS. and Mansfeld J. (2018). An E2-ubiquitin thioester-driven approach to identify substrates modified with ubiquitin and ubiquitin-like molecules. Nature Communications 9:4776

Daniel K*., Icha, J*.,  Horenburg, C., Müller D., Norden C., and Mansfeld J. (2018). Conditional control of fluorescent protein degradation by an auxin-dependent nanobody. Nature Communications 9:3297. *equal contribution                          

Grant R., Abdelbaki A., Bertoldi A., Gavilan MP., Mansfeld J., Glover DM. and Lindon C. (2018). Constitutive regulation of mitochondrial morphology by Aurora A kinase depends on a predicted cryptic targeting sequence at the N-terminus. Open Biol. 8:170272.   

Zerjatke T*., Gak I.A.*, Kirova D*., Fuhrmann M., Daniel K.,  Gonciarz M., Müller D., Glauche I., and Mansfeld J. (2017). Quantitative Cell Cycle Analysis Based on an Endogenous All-in-One Reporter for Cell Tracking and Classification. Cell Reports 19:1-14.                                    *equal contribution

Alfar E.A.*,  Kirova D*., Konantz J., Birke S., Mansfeld J#., and Ninov N#. (2017). Distinct Levels of Reactive Oxygen Species Coordinate Metabolic Activity with Beta-cell Mass Plasticity. Scientific Reports 7:3994    #corresponding authors                     *equal contribution                                                          

Gheghiani L.,  Loew D., Lombard B., Mansfeld J., and  Gavet O. (2017) PLK1 Activation in Late G2 Sets Up Commitment to Mitosis. Cell Reports 19:2060-73

Barr AR., Cooper S., Heldt FS., Butera F., Stoy H., Mansfeld J., Novák B., Bakal C. (2017). DNA damage during S-phase mediates the proliferation-quiescence decision in the subsequent G1 via p21 expression. Nature  Communications  8:14728.

Hein, MY., Hubner NC., Poser I., Cox J., Nagaraj N., Toyoda Y., Gak I., Weisswange i., Mansfeld J., Buchholz F., Hyman AA., Mann M. (2015). A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 163(3):712-23.

Otto, O., Rosendahl, P., Mietke, A., Golfier, S., Herold, C., Klaue, D., Girardo, S., Pagliara, S., Ekpenyong, A., Jacobi, A.,Wobus M., Toepfer N., Keyser UF., Mansfeld J., Fischer-Friedrich E., Guck, J. (2015). Real-time deformability cytometry: on-the-fly cell mechanical phenotyping. Nature Methods 12(3):199-202.

Di Fiore, B., Davey, N. E., Hagting, A., Izawa, D., Mansfeld, J., Gibson, T. J., and Pines, J. (2015). The ABBA Motif Binds APC/C Activators and Is Shared by APC/C Substrates and Regulators. Dev Cell 32:358–372.

Mansfeld, J., Collin, P., Collins, M. O., Choudhary, J. S., and Pines, J. (2011). APC15 drives the turnover of MCC-CDC20 to make the spindle assembly checkpoint responsive to kinetochore attachment. Nat Cell Biol 13, 1234–1243.

Mitchell, J. M., Mansfeld, J., Capitanio, J., Kutay, U., and Wozniak, R. W. (2010). Pom121 links two essential subcomplexes of the nuclear pore complex core to the membrane. J Cell Biol 191, 505–521.

Garnett, M. J., Mansfeld, J., Godwin, C., Matsusaka, T., Wu, J., Russell, P., Pines, J., and Venkitaraman, A. R. (2009). UBE2S elongates ubiquitin chains on APC/C substrates to promote mitotic exit. Nat Cell Biol 11, 1363–1369.

Sehring, I. M., Mansfeld, J., Reiner, C., Wagner, E., Plattner, H., and Kissmehl, R. (2007). The actin multigene family of Paramecium tetraurelia. BMC Genomics 8, 82.

Sehring, I. M., Reiner, C., Mansfeld, J., Plattner, H., and Kissmehl, R. (2007). A broad spectrum of actin paralogs in Paramecium tetraurelia cells display differential localization and function. J Cell Sci 120, 177–190.

Mansfeld, J., Güttinger, S., Hawryluk-Gara, L. A., Panté, N., Mall, M., Galy, V., Haselmann, U., Mühlhäusser, P., Wozniak, R. W., Mattaj, I. W., et al. (2006). The conserved transmembrane nucleoporin NDC1 is required for nuclear pore complex assembly in vertebrate cells. Mol Cell 22, 93–103.

Schilde, C., Wassmer, T., Mansfeld, J., Plattner, H., and Kissmehl, R. (2006). A multigene family encoding R-SNAREs in the ciliate Paramecium tetraurelia. Traffic 7, 440–455.


2015   ERC Starters grant

2015   TU Dresden Young Investigator

2012   DFG Emmy Noether Fellowship

2012   FEBS Distinguished Young Investigator Award

2008   FEBS long-term fellowship

2008   EMBO long-term fellowship

Group Members

Please visit our lab page to get to know us better.

You can find a list of current group members and contact information members here.

Open Positions

We are always looking for excellent and enthusiastic people. Please send your CV and why you want to join the lab.

We currently seek a highly motivated Postdoc with experience in redox biology and ideally an affinity for programming (i.e. via MATLAB, Mathematica or Python for single cell analyses).

Prospective PhD students are encouraged to apply through the International Max Planck Research School for Cell, Developmental and Systems Biology in cooperation with the Dresden International Graduate School for Biomedicine and Bioengineering. Informal inquiries are welcome.

We are currently looking for enthusiastic people with a background in bioinformatics especially in Python and R but do not mind to get their hands wet, for two projects that heavily rely on live cell imaging and single cell analyses.


CellTracking is a software to simplify fluorescence quantification in single cells during time-lapse microscopy. The standard procedure (definition of cell limits -segmentation-/tracking/cell cycle phases tagging/fluorescence export/alignment of multiple tracks) is partially automated. CellTracking is multiplatform, free, open source and can be downloaded here.CellTracking

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