Examplepictures of DNA-Structures

Jochen Guck - Cellular Machines

Cells are the basic entities of biological systems. They can be seen as little machines that have particular physical properties, which enable them to navigate their 3D physical environment and fulfil their biological functions. We investigate these physical - mechanical and optical - properties of living cells and tissues using novel photonics and biophysical tools to test their biological importance. Our ultimate goal is the transfer of our findings to medical application in the fields of improved diagnosis of diseases and novel approaches in regenerative medicine.

"Film about Jochen Guck"    Copyright: Alexander von Humboldt Foundation

Link to the Alexander von Humboldt Professorship

News

 

15. Mar 2015: Mirjam Schürmann wins 1st poster prize at the DPG Meeting in Berlin

2. Mar 2015: Full publication of new high-throughput cell mechanics measurement method "real-time deformability cytometry" (RT-DC) in Nature Methods (http://www.nature.com/nmeth/journal/v12/n3/full/nmeth.3281.html)

23. Feb 2015: RT-DC is featured in MDR Sachsenspiegel (http://www.mdr.de/mediathek/fernsehen/video254464_zc-7931f8bf_zs-2d7967f4.html)

30. Jan 2015: Philipp Rosendahl receives Georg-Helm Preis for his Diploma thesis as one of the four best theses submitted at the TU Dresden in 2014 – see also http://www.biotec.tu-dresden.de/news.html

 

 

Research

While most current biological research focuses on molecular and biochemical aspects of cells and their functioning, we are interested in their global physical properties and how the cells relate to the physical properties of their environment. 

Cell mechanics

Our findings increasingly demonstrate that the mechanical properties of cells determine the physical limits of cell function - for example in 3D cell migration. Cell mechanics can thus be used to characterise cells, to monitor physiological changes (such as stem cell differentiation), and to diagnose pathological alterations (such as metastatic progression or inflammatory reactions). Publication: F. Lautenschläger et al., The regulatory role of cell mechanics for migration of differentiating myeloid cells. Proc. Natl. Acad. Sci. U.S.A. 106:15696-15701 (2009); T.W. Remmerbach et al., Oral Cancer Diagnosis by Mechanical Phenotyping. Cancer Res. 69:1728–1732 (2009); S.M. Man et al., Actin polymerization as a key innate immune effector mechanism to control Salmonella infection. Proc. Natl. Acad. Sci. USA p. 201419925 (2014).

Constituting a quantum leap in throughput, we have just introduced Real-Time Deformability Cytometry (RT-DC) for the continuous cell mechanical characterization of large populations (>100,000 cells) with analysis rates greater than 100 cells/s. This is 4-5 orders of magnitude faster than is possible with AFM, micropipette aspiration or even optical stretcher. And the total numbers of cells analyzed is 50 times greater than with recent high-throughput developments, such as deformability cytometry (Gossett et al., PNAS, 2012).

 

Real-time deformability cytometry. (a) Schematic illustration of measurement principle (inset shows top-view of constriction). (b) Time-series of cell deformed through constriction. Scale bar, 50 µm. (c) Image of cell deformed in constriction; contour (red) according to image analysis algorithm. Scale bar, 5 µm. (d) Scatter plot of cell size vs. deformation (= 1 – circularity) of 4195 cells (dots) obtained in 45 s. Color indicates a linear density scale, black line the 50%-density contour. (e) Shear stress (left) and pressure (right) on cell surface inside constriction. Black arrows indicate stress directions, surface color the magnitude. Blue lines show the flow profile in a co-moving reference frame. Characteristic deformed shape of an elastic sphere (bottom). Color code indicates displacement relative to non-deformed sphere. (f) Iso-elasticity lines divide the size-deformation scatter plots into areas of identical stiffness for multiples of a given elastic modulus Eo.

RT-DC is sensitive to cytoskeletal alterations and can distinguish cell-cycle phases, track stem cell differentiation into distinct lineages and identify cell populations in whole blood by their mechanical fingerprints. This technique adds a new marker-free dimension to flow cytometry with diverse applications in biology, biotechnology and medicine. Publication: Otto et al., Real-time deformability cytometry: on-the-fly cell mechanical phenotypingNat. Methods, doi:10.1038/nmeth.3281 (2015) – see also: http://www.nature.com/nmeth/journal/vaop/ncurrent/full/nmeth.3281.html

A complimentary technique we have been developing for over a decade, is the optical stretcher, an established laser tool that can be used to trap and deform individual biological cells in order to test mechanical properties. With a throughput on the order of 100 cells/hour it is well suited for the detailed viscoelastic characterization of small numbers of cells.

Schematic of an optical stretcher. In a microfluidic flow chamber, cells in suspension (green) can be trapped by two opposing laser beams (red) of low intensity, emanating from optical fibers (blue). Increasing the intensity of the laser light augments the forces at the surface of the cell, leading to measurable deformation. Publication: J. Guck et al., Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence Biophys. J. 88:3689-3698 (2005)

A derivative of the dual-beam laser trap (optical stretcher) is the optical cell rotator, which can be used to trap and rotate individual cells. For more information see: Kreysing et al., Dynamic operation of optical fibres beyond single-mode regime facilitates the orientation of biological cells. Nat. Commun. 5:5481-5486 (2014).

Mechanosensing and Tissue Mechanics in the CNS

It is increasingly recognised that cells react to the mechanical properties of their environment and that these mechanical cues can be as important as adhesive or soluble biochemical cues. We are especially interested in assessing the importance of this "mechanosensing" in the development and in pathological conditions in the central nervous system. For a recent review, please see: K. Franze and J.Guck, The biophysics of neuronal regrowth Rep. Progr. Phys. 73:094601 (2010)

In order to characterise and quantify the mechanical environment of cells in the central nervous system with high spatial resolution, we have developed the mechanical mapping of CNS tissue using scanning force microscopy. Publication: A. Christ et al., Mechanical difference between white and grey matter in the rat cerebellum measured by scanning force microscopy J. Biomechanics 43:2986-2992 (2010)

Retina Optics

Another example for the importance of physics in biology are the optical properties of cells, specifically in the retina, which is, curiously, inverted with respect to its optical function. The light-sensing photoreceptor cells are located on the 'wrong' side - the side furthest away from the incoming light. Consequently, light has to traverse hundreds of microns of potentially scattering tissue. We have shown that there are cells in the retina that act as optical fibers and that photoreceptor cells even invert their usual nuclear chromatin arrangement to turn them into microlenses. Both aspects improve the light transmission through the retina and shed new light on the retina as an optical system. Publications: K. Franze et al., Müller cells are living optical fibers in the vertebrate retina. Proc. Natl. Acad. Sci. U.S.A. 104:8287-9292 (2007); I. Solovei et al., Nuclear architecture of rod photoreceptor cells adapts to vision in mammalian evolution. Cell 137:356-368 (2009).

Publications

If you are interested in a pdf of any of these publications, please contact me by email.

  1. U. Delabre, K. Feld, E. Crespo, G. Whyte, C. Sykes, U. Seifert, and J. Guck, Deformation of phospholipid vesicles in an optical stretcher. Soft Matter (accepted for publication).
  2. C. J. Chan, A. E. Ekpenyong, S. Golfier, W. Li, K. J. Chalut, O. Otto, J. Elgeti, J. Guck and F. Lautenschläger, Myosin II Activity Softens Cells in Suspension. Biophys J. 108(8):1856–69 (2015).CLICK HERE FOR PDF!
  3. M. Schürmann, J. Scholze, P. Müller, C. J. Chan, A. E. Ekpenyong, K. J. Chalut, and J. Guck, Refractive index measurements of single, spherical cells using digital holographic microscopy, in Meth. Cell Biol. 125:143–159 (2015).
  4. O. Otto, Ph. Rosendahl, A. Mietke, S. Golfier, Ch. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A. Jacobi, M. Wobus, N. Töpfner, U. F. Keyser, J. Mansfeld, E. Fischer-Friedrich, and J. Guck, Real-time deformability cytometry: on-the-fly cell mechanical phenotyping, Nat. Methods, 12(3):199-202 (2015).
  5. C. Faigle, F. Lautenschläger, G. Whyte, P. Homewood, E. Martin-Badosa, and J. Guck,  A monolithic glass chip for active single-cell sorting based on mechanical phenotyping, Lab Chip, 15:1267-1275 (2015).
  6. S. M. Man, A. Ekpenyong, P. Tourlomousis, S. Achouri, E. Cammarota, K. Hughes, A. Rizzo, G. Ng, J. A. Wright, P. Cicuta, J. R. Guck, and C. E. Bryant. Actin polymerization as a key innate immune effector mechanism to control Salmonella infection. Proc. Natl. Acad. Sci. USA p. 201419925 (2014).
  7. M. Kreysing, D. Ott, M. J. Schmidberger, O. Otto, M. Schürmann, E. Martín-Badosa, G. Whyte, and J. Guck. Dynamic operation of optical fibres beyond single-mode regime facilitates the orientation of biological cells. Nat. Commun. 5:5481-5486 (2014).
  8. D. Holmes, G. Whyte, J. Bailey, N. Vergara-Irigaray, A. Ekpenyong, J. Guck, and T Duke. Separation of blood cells with differing deformability using deterministic lateral displacement. Interface Focus 4(6), 20140011 (2014).
  9. S. Blaszczak, M. Kreysing,and J. Guck. Direct observation of light focusing by single photoreceptor cell nuclei. Opt. Express 22(9):11043-11060 (2014).
  10. P. Moshayedi, G. Ng, J. C. F. Kwok, G. S. H. Yeo, C. E. Bryant, J. W. Fawcett, K. Franze, and J. Guck. The relationship between glial cell mechanosensitivity and foreign body reactions in the central nervous system. Biomaterials 35(13):3919-3925 (2014).
  11. C.J. Chan, G. Whyte, L. Boyde, G. Salbreux, and J. Guck. Impact of heating on passive and active biomechanics of suspended cells. Interface Focus 4(2):20130069 (2014).
  12. M. Francke, M. Kreysing, A. Mack, J. Engelmann, A. Karl, F. Makarov, J. Guck, M. Kolle, H. Wolburg, and A. Reichenbach. Grouped Retinae and Tapetal Cups in some Teleostian Fish: Occurrence, Structure, and Function. Prog. Ret. Eye Res. 38:43–69 (2014).
  13. S. Kretschmer, Ch. Wolf, N. König, W. Staroske, J. Guck, M. Häusler, H. Luksch, L. A. Nguyen, B. Kim, D. Alexopoulou, A. Dahl, A. Rapp, M. C. Cardoso, A. Shevchenko, M. A. Lee-Kirsch. SAMHD1 prevents autoimmunity by maintaining genome stability. Ann. Rheum. Dis. doi:10.1136/annrheumdis-2013-204845 (2014).
  14. J. Guck and E. R. Chilvers. Mechanics Meets Medicine. Sci. Transl. Med. 5, 212fs41 (2013). CLICK HERE FOR A REPRINT!
  15. K. Franze, P. A. Janmey, and J. Guck. Mechanics in Neural Development and Repair. Annu. Rev. Biomed. Eng. 15:227–51 (2013).
  16. A. E. Ekpenyong, S. M. Man, S. Achouri, C. E. Bryant, J. Guck, and K. J. Chalut. Bacterial infection of macrophages induces decrease in refractive index. J. Biophot. 6(5):393-397 (2013).
  17. K. J. Chalut, M. Höpfler, F. Lautenschläger, L. Boyde, C. J. Chan, A. Ekpenyong, A. Martinez-Arias, and J. Guck. Chromatin Decondensation and Nuclear Softening Accompany Nanog Downregulation in Embryonic Stem Cells. Biophys. J. 103(10):2060–2070 (2012).
  18. L. Boyde, A. E. Ekpenyong, G. Whyte, and J. Guck. Elastic Theory for the Deformation of a Solid or Layered Spheroid under Axisymmetric Loading. Acta Mechanica  doi: 10.1007/s00707-012-0789-7 (2012).
  19. L. Boyde, A. E. Ekpenyong, G. Whyte, and J. Guck. Comparison of Stresses on Homogeneous Spheroids in the Optical Stretcher Computed with Geometrical Optics and Generalised Lorenz-Mie Theory. Appl. Opt. 51(33):7934-7944 (2012).
  20. A. E. Ekpenyong, G. Whyte, K. Chalut, S. Pagliara, F. Lautenschläger, C. Fiddler, S. Paschke, U. F. Keyser, E. R. Chilvers, and J. Guck. Viscoelastic Properties of Differentiating Blood Cells are Fate- and Function-Dependent. PLoS One 7(9):e45237 (2012).
  21. A. E. Ekpenyong, S. M. Man, S. Achouri, C. E. Bryant, J. Guck, and K. J. Chalut. Bacterial infection of macrophages induces decrease in refractive index. J. Biophot. doi:10.1002/jbio.201200113.
  22. H. K. Matthews, U. Delabre, J. L. Rohn, J. Guck, P. Kunda, and B. Baum. Changes in Ect2 Localization Couple Actomyosin-dependent Cell Shape Changes to Mitotic Progression. Dev. Cell 23(2):371-383 (2012).
  23. M. Kreysing, R. Pusch, D. Haverkate, M. Landsberger, J. Engelmann, J. Ruiter, C. Mora-Ferrer, E. Ulbricht, J. Grosche, K. Franze, S. Streif, S. Schumacher, F. Makarov, J. Kacza, J. Guck, H. Wolburg, J.K. Bowmaker, G. von der Emde, S. Schuster, H.-J. Wagner, A. Reichenbach, and M. Francke. Photonic crystal light collectors in fish retina improve vision in turbid water. Science 336(6089):1700–1703 (2012).
  24. A. Jagielska, A. Norman, G. Whyte, K. J. Van Vliet, J. Guck, and R. Franklin. Mechanical environment modulates biological properties of oligodendrocyte progenitor cells. Stem Cells Dev. 21(16):2905-2914 (2012).
  25. P. K. Trong, J. Guck, and R. E. Goldstein. Coupling of active motion and advection shapes intracellular cargo transport. Phys. Rev. Lett. 109:028104 (2012).
  26. A. Reichenbach, K. Franze, S. Agte, S. Junek, A. Wurm, J. Grosche, A. Sawinov, J. Guck, and S. N. Skatchkov. Live Cells as Optical Fibers in the Vertebrate Retina, in: Selected Topics on Optical Fiber Technology, ed. by M. Yasin, H. Arof, and S. W. Harun, InTech, Rijeka (2012).
  27. K. J. Chalut, A. E. Ekpenyong, W. L. Clegg, I. C. Melhuish, and J. Guck. Quantifying cellular differentiation by physical phenotype using digital holographic microscopy. Integr. Biol. 4(3):280-284 (2012).
  28. J. da Silva, F. Lautenschläger, C.-H. R. Kuo, J. Guck and E. Sivaniah. 3D inverted colloidal crystals in realistic cell migration assays for drug screening applications. Integr. Biol. 3(12):1202-1206 (2011).
  29. L. Boyde, K.J. Chalut, and J. Guck. Exact analytical expansion of an off-axis Gaussian laser beam using the translation theorems for the vector spherical harmonics. Appl. Opt. 50(7):1023-1033 (2011).
  30. L. Boyde, K.J. Chalut, and J. Guck. Near- and far-field scattering from arbitrary three-dimensional aggregates of coated spheres using parallel computing. Phys. Rev. E 83:026701 (2011).
  31. K. Franze, M. Francke, K. Günter, A.F. Christ, N. Körber, A. Reichenbach, and J. Guck. Spatial mapping of the mechanical properties of the living retina using scanning force microscopy. Soft Matter, 7(7):3147–3154 (2011).
  32. J. Guck. Cell Sorting, in: Handbook of Biophotonics, Vol. 2: Photonics for Health Care, ed. by J. Popp, V.V. Tuchin, A. Chiou, and S. Heinemann, Wiley-VCH, Berlin (2011).
  33. J.M. Maloney, D. Nikova, F. Lautenschläger, E. Clarke, R. Langer, J. Guck, and K.J. Van Vliet. Mesenchymal stem cell mechanics from the attached to the suspended state. Biophys. J. 99(8):2479-2487 (2010).
  34. K. Franze and J. Guck. The biophysics of neuronal regrowth. Rep. Prog. Phys. 73:094601 (2010).
  35. J. Guck, F. Lautenschläger, S. Paschke, and M. Beil. Critical review: Cellular mechanobiology and amoeboid migration. Integr. Biol. 2(11):575-583 (2010).
  36. A.F. Christ, K. Franze, H. Gautier, P. Moshayedi, J. Fawcett, R.J.M. Franklin, R.T. Karadottir, and J. Guck. Mechanical difference between white and gray matter in the rat cerebellum measured by scanning force microscopy. J. Biomech. 43:2986–2992 (2010).
  37. M. Kreysing, L. Boyde, J. Guck, and K. Chalut. Physical insight into light scattering by photoreceptor cell nuclei. Opt. Lett. 35(15):2639-2641 (2010).
  38. J.M.A Mauritz, A. Esposito, T. Tiffert, J.N. Skepper, A. Warley, Y.Z. Yoon, P. Cicuta, R., V.L. Lew, J. Guck, and C.F. Kaminski. Biophotonic techniques for the study of malaria-infected red blood cells. Med. Biol. Engin. Comp. 48(10): 1055-1063 (2010).
  39. J.M.A Mauritz, T. Tiffert, R. Seear, F. Lautenschläger, A. Esposito, V.L. Lew, J. Guck, and C.F. Kaminski. Detection of Plasmodium falciparum-infected red blood cells by optical stretching. J. Biomed. Opt. 15:030517 (2010).
  40. B. Kemper, P. Langehanenberg, A. Höink, G. von Bally, F. Wottowah, S. Schinkinger, J. Guck, J. Käs, I. Bredebusch, J. Schnekenburger, and K. Schütze. Monitoring of Laser Micromanipulated Optically Trapped Cells by Digital Holographic Microscopy. J. Biophot. 3(7):425-431 (2010).
  41. P. Moshayedi, L. da F Costa, A. Christ, S. P. Lacour, J. Fawcett, J. Guck, and K. Franze. Mechanosensitivity of astrocytes on optimized polyacrylamide gels analyzed by quantitative morphometry. J. Phys.: Cond. Matter 22(19):194114 (2010).
  42. J. da Silva, F. Lautenschläger, E. Sivaniah, and J. Guck. The cavity-to-cavity migration of leukaemic cells through 3D honey-combed hydrogels with adjustable internal dimension and stiffness. Biomaterials 31(8):2201–2208 (2010).
  43. I. Solovei, M. Kreysing, Ch. Lanctôt, S. Kösem, L. Peichl, Th. Cremer, J. Guck*, and B. Joffe*. Nuclear architecture of rod photoreceptor cells adapts to vision in mammalian evolution. Cell 137(2):356-68 (2009) [* joint corresponding authors].
  44. J. Guck. Do cells care about physics? Physics World 22(7): 31-34 (2009).
  45. F. Lautenschläger, S. Paschke, A. Bruel, S. Schinkinger, M. Beil, and J. Guck. The regulatory role of cell mechanics for migration of differentiating myeloid cells. Proc. Natl. Acad. Sci. U.S.A. 106(37):15696-15701 (2009).
  46. L. Boyde, K. Chalut, and J. Guck. Interaction of Gaussian Beam with Near-Spherical Particle: an Analytic-Numerical Approach for Assessing Scattering and Stresses. JOSA A 26(8):1814–26 (2009).
  47. T.W. Remmerbach, F. Wottawah, J. Dietrich, B. Lincoln, Ch. Wittekind, and J. Guck. Oral Cancer Diagnosis by Mechanical Phenotyping. Cancer Res. 69(5):1728–1732 (2009).
  48. M. K. Kreysing, T. Kießling, A. Fritsch, Ch. Dietrich, J. R. Guck, J. A. Käs. The optical cell rotator. Opt. Express 16(21): 16984-16992 (2008).
  49. K. Franze, J. Grosche, S. N. Skatchkov, S. Schinkinger, D. Schild, O. Uckermann, K. Travis, A. Reichenbach, and J. Guck. Müller Cells are Living Optical Fibers in the Vertebrate Retina. Proc. Natl. Acad. Sci. U.S.A. 104(20):8287-8292 (2007).
  50. B. Lincoln, S. Schinkinger, F. Wottawah, and J. Guck. High-Throughput Cell Analysis with a Microfluidic Optical Stretcher. Meth. Cell Biol. 83:397-423 (2007).
  51. B. Lincoln, S. Schinkinger, K. Travis, F. Wottawah, S. Ebert, F. Sauer, and J. Guck. Reconfigurable microfluidic integration of a dual-beam laser trap with biomedical applications. Biomed. Microdev. 9(5):703-710 (2007).
  52. S. Ebert, K. Travis, B. Lincoln, J. Guck. Fluorescence Thermometry in a Microfluidic Dual-Beam Laser Trap. Opt. Express 15(23):15493-15499 (2007).
  53. R. Ananthakrishnan, J. Guck, F. Wottawah, S. Schinkinger, B. Lincoln, M. Romeyke, T. Moon, and J. Käs. Quantifying the Contribution of Actin Networks to the Elastic Strength of Fibroblasts. J. Theo. Biol. 242:502–516 (2006).
  54. Y. Lu, K. Franze, G. Seifert, Ch. Steinhäuser, F. Kirchhoff, H. Wolburg, J. Guck, P. Janmey, E. Wei, J. Käs, and A. Reichenbach. Viscoelastic properties of individual glial cells and neurons in the CNS. Proc. Natl. Acad. Sci. U.S.A. 103(47):17759–177764 (2006).
  55. R. Ananthakrishnan, J. Guck, and J. Käs. Cell mechanics: Recent advances with a theoretical perspective, in: Recent Research Developments in Biophysics, Transworld Research Network 5:39-69 (2006)
  56. K. Travis and J. Guck. Scattering from Single Nanoparticles: Mie theory revisited. Biophys. Rev. Lett. 1(2):115–143 (2006).
  57. F.-U. Gast, P. S. Dittrich, P. Schwille, M. Weigel, M. Mertig, J. Opitz, U. Queitsch, S. Diez, B. Lincoln, F. Wottawah, S. Schinkinger, J. Guck, J. Käs, J. Smolinski, K. Salchert, C. Werner, C. Duschl, M. S. Jäger, K. Uhlig, P. Geggier, S. Howitz. The microscopy cell (MicCellTM), a versatile modular flowthrough system for cell biology, biomaterial research, and nanotechnology. Microfluid. Nanofluid. 2(1):21–36 (2006).
  58. F. Wottawah, S. Schinkinger, B. Lincoln, R. Ananthakrishnan, M. Romeyke, J. Guck, and J. Käs. Optical Rheology of Biological Cells. Phys. Rev. Lett. 94(9):98103 (2005)
  59. F. Wottawah, S. Schinkinger, B. Lincoln, S. Ebert, K. Müller, F. Sauer, K. Travis, and J. Guck. Characterizing Single Suspended Cells by Optorheology. Acta Biomat. 1:263–271 (2005).
  60. J. Guck, H. Erickson, R. Ananthakrishnan, D. Mitchell, M. Romeyke, S. Schinkinger, F. Wottawah, B. Lincoln, J. Käs, S. Ulvick, and C. Bilby. Optical Deformability as Inherent Cell Marker for Testing Malignant Transformation and Metastatic Competence. Biophys. J. 88(5):3689-3698 (2005).
  61. R. Ananthakrishnan, J. Guck, F. Wottawah, S. Schinkinger, B. Lincoln, M. Romeyke, T.J. Moon, and J Käs. Modelling the structural response of an eukaryotic cell in the optical stretcher. Curr. Sci. 88(9):1434–1440 (2005).
  62. T. Betz, J. Teipel, D. Koch, J. Guck, J. Käs, H. Gießen. Excitation beyond the monochromatic laser limit: Simultaneous 3-D confocal and multiphoton microscopy with a tapered fiber as white-light laser source. J. Biomed. Opt. 10(5):054009 (2005)
  63. B. Lincoln, H. M.Erickson, S. Schinkinger, F. Wottawah, D. Mitchell, S. Ulvick, C. Bilby, and J. Guck. Deformability-Based Flow Cytometry. Cytometry A 59:203-209 (2004).
  64. S. Schinkinger, K.A. Travis, F. Wottawah, B. Lincoln, and J. Guck. Feeling for Cells with Light. Proc. SPIE: Optical Trapping and Optical Micromanipulation 5514:170-178 (2004).
  65. J. Guck, R. Ananthakrishnan, C.C. Cunningham, and J. Käs. Stretching Biological Cells with Light. J. Phys. Cond. Mat. 14: 4843-4856 (2002).
  66. J. Guck, R. Ananthakrishnan, H. Mahmood, T.J. Moon, C.C. Cunningham, and J. Käs. The Optical Stretcher: A Novel Laser Tool to Micromanipulate Cells. Biophys. J. 81(2):767–784 (2001).
  67. J. Guck. Optical Deformability–Micromechanics from Cell Research to Biomedicine. Ph.D. dissertation University of Texas at Austin, U.S.A. (2001).
  68. J. Guck, R. Ananthakrishnan, T.J. Moon, C.C. Cunningham, and J. Käs. Optical Deformability of Soft Biological Dielectrics. Phys. Rev. Lett. 84(23):5451-5454 (2000)
  69. J. Käs, J. Guck, and D. Humphrey, Dynamics of Single Protein Polymers Visualized by Fluorescence Microscopy, in: Modern Optics, Electronics and High Precision Techniques in Cell Biology, ed. by G. Isenberg, Springer Verlag, 103-137 (1997).

Curriculum Vitae

  • 2012 Professor of Cellular Machines, Biotechnology Center, TU-Dresden
  • 2012 Alexander von Humboldt Professor
  • 2012 Principal research scientist, University of Cambridge
  • 2009 Reader, Cavendish Laboratory, University of Cambridge
  • 2007 Lecturer, Cavendish Laboratory, University of Cambridge
  • 2002 Junior group leader, Universität Leipzig
  • 2001 PhD in Physics, University of Texas at Austin

Positions

There are currently two PhD positions available. Both are part of a Marie Curie ITN on "Biochemical and mechanochemical signaling in polarized cells – BIOPOL". More information (positions P11 and P13) can be found here: http://www.sheffield.ac.uk/itn-biopol/positions

Applicants for ERC Starting Grants and DFG Emmy Noether Fellowships in relevant scientific areas are encouraged to contact me to discuss ideas, and for grant writing support, lab space and leveraging funds.

We are also welcoming inquiries into sabbaticals in our group. Some funding is available to support such endeavors.

Frontcovers


  

Group Members

You can find a list of current group members here.

Physics-Proseminar "Physics aspects in biology"

Physics-Proseminar "Physics aspects in biology"

  • SS 2015 (Bachelor, 4. Semester)
  • Time/Location: DO(3) BIOTEC
  • Prof. Dr. Jochen Guck
  • Contact: jochen.guck(at)biotec.tu-dresden.de
  • Inscription deadline: Friday, 10. April 2015  by EMAIL
  • Further information: Please send an email with a ranked list of 2-3 topics by this date to one of the contact emails above. The topics will be assigned and communicated by return email within two weeks. In general, you should contact me at least one week ahead of your date with a detailed outline of your presentation. Talks will be 30 min + 15 min scientific discussion and feedback. The language of the course is English.

 

Possible topics and literature suggestions:

 

1.  DNA Biomechanics

  • Milstein, J. N., & Meiners, J.-C. (2011). On the role of DNA biomechanics in the regulation of gene expression. J R Soc Interface 8:1673-1681. [pdf]
  • Bustamante, C., Cheng, W., & Meija, Y. X. (2011). Revisiting the Central Dogma One Molecule at a Time. Cell144(4), 480-497. [pdf]
  • Bustamante, C., Bryant, Z., & Smith, S. B. (2003). Ten years of tension: single-molecule DNA mechanics. Nature421, 423-427. [pdf]

2. Optical traps in biology

  • Svoboda, K. and S. M. Block (1994). "Biological applications of optical forces." Annu Rev Biophys Biomol Struct 23: 247-285. [pdf]
  • Keyser, U. F., Koeleman, B. N., van Dorp, S., Krapf, D., Smeets, R. M. M., Lemay, S. G., Dekker, N. H., et al. (2006). Direct force measurements on DNA in a solid-state nanopore. Nature Physics2(7), 473-477. [pdf]
  • Keyser, U. F., van Dorp, S., & Lemay, S. G. (2010). Tether forces in DNA electrophoresis. Chemical Society reviews39(3), 939-947. [pdf]

3. Single-Molecule Micromanipulation Techniques

  • Neuman, K. C., Lionnet, T., & Allemand, J.-F. (2007). Single-Molecule Micromanipulation Techniques. Annual Review of Materials Research37(1), 33-67. [pdf]
  • Müller, D.J., Helenius, J., Alsteens, D. & Dufrêne, Y.F. (2009). Force probing surfaces of living cells to molecular resolution. Nature Chemical Biology 5, 383 - 390. [pdf]

4. Forces and bond dynamics in cell adhesion

  • Thomas W. (2008). Catch Bonds in Adhesion. Annu. Rev. Biomed. Eng10, 39-57 . [pdf]
  • Evans, E. A. & Calderwood, D. A. (2007). Forces and Bond Dynamics in Cell Adhesion. Science 316, 1148. [pdf]
  • Leckband D. (2000). Measuring the forces that control protein interactions. Annu. Rev. Biophys. Biomol. Struct29, 1-26. [pdf

5. The optical stretcher: a novel laser tool to micromanipulate cells.

  • Guck, J., Ananthakrishnan, R., Moon, T. J., Cunningham, C. C., & Käs, J. (2000). Optical deformability of soft biological dielectrics. Phys Rev Lett84(23), 5451–5454. [pdf]
  • Guck, J., Ananthakrishnan, R., Mahmood, H., Moon, T. J., Cunningham, C. C., & Käs, J. (2001). The optical stretcher: a novel laser tool to micromanipulate cells. Biophysical Journal81(2), 767-784. [pdf]
  • Ekpenyong, A. E., Whyte, G., Chalut, K., Pagliara, S., Lautenschlaeger, F., Fiddler, C., & Guck, J. (2012). Viscoelastic Properties of Differentiating Blood Cells Are Fate- and Function-Dependent. PLOS one7(9), 45237. [pdf]

6. Hydrodynamic single cell classification

  • Goda, K., Ayazi, A., Gossett, D. R., Sadasivam, J., Lonappan, C. K., Sollier, E., et al. (2012). High-throughput single-microparticle imaging flow analyzer. Proceedings of the National Academy of Sciences of the United States of America109(29), 11630. [pdf]
  • Wu, M., Perroud, T. D., Srivastava, N., Branda, C. S., Sale, K. L., Carson, B. D.,  et al. (2012). Microfluidically-unified cell culture, sample preparation, imaging and flow cytometry for measurement of cell signaling pathways with single cell resolution. Lab on a chip12(16), 2823–31. [pdf]
  • Hur, S. C., Henderson-MacLennan, N. K., McCabe, E. R. B., & Di Carlo, D. (2011). Deformability-based cell classification and enrichment using inertial microfluidics. Lab on a chip11(5), 912–20. [pdf]

7. The physics of cancer

  • Wirtz, D., Konstantopoulos, K., & Searson, P. C. (2011). The physics of cancer: the role of physical interactions and mechanical forces in metastasis. Nature reviews Cancer11, 512–522. [pdf]
  • Guck, J., Schinkinger, S., Lincoln, B., Wottawah, F., Ebert, S., Romeyke, M., Lenz, D., et al. (2005). Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence. Biophysical Journal88(5), 3689–3698. [pdf]
  • Plodinec M., Loparic M., Monnier C. A., Obermann E. C., Zanetti-Dallenbach R., Oertle P., Hyotyla J. T., Aebi U., Bentires-Alj M., Lim R. Y., Schoenenberger C. A. (2012). The nanomechanical signature of breast cancer. Nat Nanotechnol7(11),757-65. [pdf]

8. How cells feel and respond to the mechanical properties of their environment

  • Discher, D. E., Janmey, P., & Wang, Y.-L. (2005). Tissue Cells Feel and Respond to the Stiffness of Their Substrate. Science310(5751), 1139–1143. [pdf]
  • Wozniak, M. A., & Chen, C. S. (2009). Mechanotransduction in development: a growing role for contractility. Nature Reviews Molecular Cell Biology10(1), 34–43. [pdf]
  • Marcq, P., Yoshinaga, N., & Prost, J. (2011). Rigidity Sensing Explained by Active Matter Theory. Biophysical Journal101(6), L33–L35. [pdf]

9. Life at small Reynolds number

  • Purcell, E. (1977). Life at Low Reynolds-Number. American Journal of Physics45(1), 3–11. [pdf]

10. Bacterial chemotaxis

  • Berg, H. C., E. Coli in Motion (Springer, NY, 2004) 133pp.
  • Berg, H. C., Motile behaviour of bacteria. Physics Today, 53(1), 24-29. [pdf]
  • Alon, U., Surette, M. G., Barkai, N., & Leibler, S. (1999). Robustness in bacterial chemotaxis. Nature397(6715), 168–171. [pdf]

11. Pattern formation in biological systems

  • Koch, A. J., & Meinhardt, H. (1994). Biological pattern formation: from basic mechanisms to complex structures. Reviews of Modern Physics66(4), 1481–1507. [pdf]

12. Super-resolution microscopy: principles and use in biology

  • Schermelleh, L., Heintzmann, R., & Leonhardt, H. (2010). A guide to super-resolution fluorescence microscopy. J Cell Biol190(2), 165–175. [pdf]
  • Huang, B., Bates, M., & Zhuang, X. (2009). Super-Resolution Fluorescence Microscopy. Annual Review of Biochemistry78(1), 993–1016.

13. The physics of hearing

  • Bell, A. (2004). Hearing: Travelling Wave or Resonance? PLoS Biology2(10), e337. [pdf]
  • Plaçais, P. Y., Balland, M., Guérin, T., Joanny, J. F., & Martin, P. (2009). Spontaneous Oscillations of a Minimal Actomyosin System under Elastic Loading. Phys Rev Lett103(15). [pdf]
  • Camalet, S., Duke, T., Jülicher, F., & Prost, J. (2000). Auditory sensitivity provided by self-tuned critical oscillations of hair cells. Proceedings of the National Academy of Sciences of the United States of America97(7), 3183–3188. [pdf]

14. Kinetic proofreading: why there are not more mistakes in copying DNA

  • Hopfield, J. J. (1974). Kinetic proofreading: a new mechanism for reducing errors in biosynthetic processes requiring high specificity. Proceedings of the National Academy of Sciences of the United States of America71(10), 4135–4139. [pdf]
  • Yan, J., Magnasco, M. O., & Marko, J. F. (1999). A kinetic proofreading mechanism for disentanglement of DNA by topoisomerases. Nature401(6756), 932–935. [pdf]

15. Physical limits on the size of cells – and why it pays to get together

  • Short, M. B., Solari, C. A., Ganguly, S., Powers, T. R., Kessler, J. O., & Goldstein, R. E. (2006). Flows driven by flagella of multicellular organisms enhance long-range molecular transport. Proceedings of the National Academy of Sciences of the United States of America103(22), 8315–8319. [pdf]
  • Solari, C. A., Ganguly, S., Kessler, J. O., Michod, R. E., & Goldstein, R. E. (2006). Multicellularity and the functional interdependence of motility and molecular transport. Proceedings of the National Academy of Sciences of the United States of America103(5), 1353. [pdf]

16. How does a cell find its middle?

  • Tran, P., Marsh, L., Doye, V., Inoue, S., & Chang, F. (2001). A mechanism for nuclear positioning in fission yeast based on microtubule pushing. J Cell Biol153(2), 397–412. [pdf]
  • Howard, M. & Kruse, K. (2005). Cellular organization by self-organization: mechanisms and models for Min protein dynamics. J Cell Biol168(4), 533–536. [pdf]

17. How nerve signals propagate

  • Biological Physics: Energy, Information, Life, Nelson P (WH Freeman 2003).
  • Heimburg, T. (2009). Die Physik von Nerven. Physik J8(3), 33–39. [pdf]

18. Brownian ratchets in biology

  • Bier, M. (1997). Brownian ratchets in physics and biology. Contemporary Physics38(6), 371–379. [pdf]
  • Reimann, P., & Hänggi, P. (2002). Introduction to the physics of Brownian motors. Applied Physics A75(2), 169–178. [pdf]
  • Peskin, C., Odell, G., & Oster, G. (1993). Cellular motions and thermal fluctuations: the Brownian ratchet. Biophysical Journal65, 316–324. [pdf]
  • Astumian, R. D. (1997). Thermodynamics and Kinetics of a Brownian Motor. Science276(5314), 917–922. [pdf]

 

General literature for the biological background:

  • Essential Cell Biology, Alberts B et al. (Garland 2003).

 Materials Summer Semester 2012 [Login]

Lecture "Introduction to Biophysics"

"Introduction to Biophysics"

  • WS 2014/15 (obligatorische Vorlesung im Wahlpflichtvertiefungsgebiet "Soft condensed matter and biological physics")
  • Also BIOTEC BioMolEng Master course "Principles of Biophysics"
  • Also BIOTEC NanoBio Maser course "Biophysical Chemistry"
  • Location: CRTD, Auditorium (right)
  • Time: Mondays, 17:00 - 18:30h - first lecture on 13.10.14
  • Prof. Dr. Jochen Guck
  • Contact: jochen.guck@tu-dresden.de
  •  The language of this lecture is English.
Content:

This lecture is an introduction to the physics of biological systems at the molecular and cellular level. In addition to providing all the basic information on this topic, the emphasis is on the physical design principles that living systems use to accomplish various cellular processes, enabling them to sense and react to their environment. A set of case studies aims to demonstrate how physicists' experience of the complex systems can complement experimental investigations by biologists to explain how living systems work, and why biology is the way it is.

  • Cells: What's in a cell? Component molecules. Cellular processes. Significance of Brownian motion, noise and stochasticity.
  • Information and Regulation: DNA replication. RNA, transcription and translation. Promotors, repressors and operons, DNA topology. Gene regulatory networks.
  • Energy: Chemiosmotic theory. Membrane potential, Nernst relation, ion channels and pumps. Metabolism and the synthesis of ATP.
  • Structural elements: The cytoskeleton: mictrotubles, actin filaments, networks and gels.
  • Cell movements and locomotion. Mechanosensing.
  • Molecular machines: Motor proteins and isothermal ratchets. Mechanochemistry and the Kramers equation. Muscle contraction. Processive motors. Rotary motors.
  • Sensory cells: Hair cells in the ear. Active signal detection and cochlear mechanics. Phototransduction in the retina.
  • Nerve impulses: Axons and the action potential. Hodgkin-Huxley model. Spiking and bursting.
Literature:
  • Biological Physics: Energy, Information, Life, Nelson P (WH Freeman 2003).
  • Physical Biology of the Cell, Phillips, Kondev, Theriot (Garland Science, 2009)
  • Cell Movements, Bray D (Garland 2000).
  • Mechanics of Motor Proteins and the Cytoskeleton, Howard J (Sinauer 2000).
  • Essential Cell Biology, Alberts B et al. (Garland 2003)

Lab course "Biophysical methods"

Lab Course "Biophysical Methods"

  • BIOTEC NanoBio Master students
  • Time: 9. – 13. March 2015
  • BIOTEC BioMolEng Master students
  • Time: 23. – 27. March 2015
  • Physics Master students, SS 2015 (obligatorische Veranstaltung im Physik Master Wahlpflichtvertiefungsgebiet "Soft condensed matter and biological physics")
  • Time: probably 16. – 20. March 2015 (to be confirmed)
  • Prof. Dr. Jochen Guck
  • Contact: jochen.guck@tu-dresden.dephillip.rosendahl@biotec.tu-dresden.de
  • MORE INFORMATION TO FOLLOW CLOSER TO THE RESPECTIVE DATES!

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