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Denis Corbeil - Tissue Engineering from Prominin-1/CD133+ stem and progenitor cells

  • 1994: Ph.D. University of Montreal, Canada 
  •  1994-1997: Postdoctoral work at the Department of Neurobiology, University of Heidelberg
  •  1997-2001: Staff Scientist at the Department of Neurobiology, University of Heidelberg
  •  2001-2004: Group Leader at Medical Clinic and Polyclinic I of TU Dresden  with joint appointment at MPI-CBG
  •  2004-present: Group Leader at Biotechnology Center of the TU Dresden 
  • 2014 – present: Honorary University Fellow, Plymouth University Peninsula Schools of Medicine & Dentistry



Previous and Current Research

The focus of our research is to understand the first step of tissue formation, which relies on the cell biological basis of stem cell proliferation and differentiation. We particularly concentrate on stem cells that express the marker prominin-1 (CD133).

Fig. 1 - Membrane topology of prominin-1 (CD133).

 Previously, we have reported the molecular and cell biological characterization of prominin-1, a pentaspan membrane glycoprotein (Fig. 1). This cholesterol-binding protein is specifically associated with plasma membrane protrusions, irrespective of the cell type, by a molecular mechanism that involves a membrane lipid microdomain. We have identified also a second prominin molecule (referred to as prominin-2) that exhibits a similar, but not identical, tissue distribution and subcellular localization to prominin-1. Moreover, several splice variants of prominin-1 have been identified and characterized. They show a broad range of expression, from myelin to the tail of spermatozoa. Importantly, prominin-1 is expressed in several stem cells originating from various sources, including the neural and hematopoietic system, and prominin-1 is now used for stem cell isolation. Likewise, certain epitopes, e.g. AC133, of prominin-1 might be used as markers of cancer stem cells.
 The physiological function of these pentaspan membrane glycoproteins, which are conserved through metazoan evolution, remains to be established. Nevertheless, the general preference of these proteins for plasma membrane protrusions, including the membrane evaginations at the based of the outer segment of photoreceptor cells and the identification of mutations in the human PROM-1 gene that cause retinal degeneration, have led to the hypothesis that prominin-1 acts as an organizer of plasma membrane protrusions.

Fig. 2 - Hematopoietic stem cells growing on multipotent mesenchymal stromal cells as feeder cell layer.

Future Prospects and Goals

In the future, we will functionally characterize prominins using molecular and cell biological approaches as well as animal models (mouse, zebrafish, axolotl). Besides studying the cell biological basis of tissue formation, in particular in the hematopoietic system (see Fig. 2 and Fig. 3), my lab will also investigate the cellular and molecular mechanisms underlying the retinal degeneration associated with mutations in the PROM-1 gene, and evaluate the possibility of using prominin-1 as a novel tool in the diagnosis of retinal degeneration.

Fig. 3 - Symmetric and asymmetric distribution of prominin-1 (AC133 epitope) in dividing hematopoietic stem cells (for details see Fargeas et al., (2006) Future Lipidology; Bauer et al., (2008) Cells Tissues Organs; Fonseca et al., (2008) Blood Cells Mol Dis). White arrows indicate the midbody. Bars, 5 µm.

 Finally, the role of prominins in lipid raft membrane microdomain formation will be investigated, as well as the function of these microdomains in normal prominin-1+ hematopoietic stem cells and their leukemic counterparts.


Prominin-1 (CD133): New Insights on Stem & Cancer Stem Cell Biology
Advances in Experimental Medicine and Biology, Vol. 777
Corbeil, Denis (Ed.) Springer Medline 


Münsterberg, J., D. Loreth, L. Brylka, S. Werner, J. Karbanová, M. Gandrass, S. Schneegans, K. Besler, F. Hamester, J. Ramon Robador, A.T. Bauer, S. Werner Schneider, M. Wrage, K. Lamszus, J. Matschke, Y. Vashist, G. Uzunoglu, S. Steurer, A.K. Horst, L. Olivera-Ferrer, M. Glatzel, T. Schinke, D. Corbeil, K. Pantel, C. Maire, H. Wikman.  (2020) ALCAM contributes to brain metastasis formation in non-small-cell lung cancer through interaction with vascular endothelium. Neuro Oncol. In press Medline

Bobinger, T., S.S. Roeder, M.I. Spruegel, K. Froehlich, V.D. Beuscher, P. Hoelter, H. Lücking, D. Corbeil and H.B. Huttner. (2020) Variation of membrane particle-bound CD133 in cerebrospinal fluid of patients with subarachnoid and intracerebral hemorrhage. J. Neurosurg. In press. Medline

Fargeas, C.A., J. Karbanová, D. Corbeil. (2020) Assessment of CD133-positive extracellular membrane vesicles in pancreatic cancer ascites and beyond. Med. Mol. Morphol. In press DOI org/10.1007/s00795-019-00221-w Medline

Corbeil, D. and A. Lorico. (2020) Exosomes, Microvesicles, and their Friends in Solid Tumors. In Exosomes in Health and Disease. A Research and Clinical Compendium. Edited by J.R. Smythies, L.R. Edelstein, P.J. Quesenberry and D. Noble. Elsevier Company. Chapter 3: 39-80 Medline


Singer, D., K. Thamm, H. Zhuang, J. Karbanová, Y. Gao, J.V. Walker, H. Jin, X. Wu, C.R. Coveney, P. Marangoni, D. Lu, P.R. Clare Grayson, T. Gulsen, K.J. Liu, S. Ardu, A. Wann, S. Luo, A.C. Zambon, A.M. Jetten, C. Tredwin, O.D. Klein, M. Attanasio, P. Carmeliet, W.B. Huttner, D. Corbeil§ and B. Hu.(§ Corresponding author).(2019) Prominin-1 governs stem cell activation by orchestrating ciliary dynamics.EMBO J. 38: pii; e99845 In press Medline

Thamm, K., D. Šimaitė, J. Karbanová, V. Bermúdez, D. Reichert, A. Morgenstern, M. Bornhäuser, W.B. Huttner, M. Wilsch-Bräuninger and D. Corbeil.(2019) Prominin-1 (CD133) modulates the architecture and dynamics of microvilli. Traffic. 20: 39-60 In press Medline

Corbeil D., C.A. Fargeas and J. Jászai. (2019) Deciphering the roles of prominins in the visual system. J. Biol. Chem. 294: 17166 Medline

Karbanová, J., D. Corbeil§ and C.A. Fargeas. (§ Corresponding author). (2019) Prominin-1/CD133, saliva and salivary glands – Integrating existing data to new clinical approaches. Exp. Cell Res. 383: 111566 Medline

Walker J.V., H. Zhuang, D. Singer, C. Illsley, W.L. Kok, K.K. Sivaraj, Y. Gao, C. Bolton, Y. Liu, M. Zhao, P.R.C. Grayson, S. Wang, J. Karbanová, T. Lee, S. Ardu, Q. Lai, J. Liu, M. Kassem, S. Chen, K. Yang, Y. Bai, C. Tredwin, A.C. Zambon, D. Corbeil, R. Adams, B. M. Abdallah, B. Hu. (2019) Transit amplifying cells coordinate mouse incisor mesenchymal stem cell activation. Nature Commun. 10: 3596 (1-18) Medline

Santos M.F., G. Rappa, J. Karbanová, C. Vanier, C. Morimoto, D. Corbeil§ and A. Lorico. (§ Corresponding author). (2019) Anti-human CD9 antibody Fab fragment impairs the internalization of extracellular vesicles and the nuclear transfer of their cargo proteins. J. Cell Mol. Med. 23: 4408-4421 Medline


Santos, M.F., G. Rappa, J. Karbanová, T. Kurth, D. Corbeil§ and A. Lorico (§ Corresponding author). (2018) VAMP-associated protein-A and oxysterol-binding protein–related protein 3 promote the entry of late endosomes into the nucleoplasmic reticulum. J. Biol. Chem. 293: 13834-13848 Medline
Karbanová, J., A. Lorico, M. Bornhäuser, D. Corbeil§ and C.A. Fargeas. (§ Corresponding author). (2018) Prominin-1/CD133: Lipid Raft Association, Detergent Resistance and Immunodetection. Stem Cells Transl Med. 7: 155-160 Medline

Maehara R., K. Fujikura, K. Takeuchi, M. Akita, S. Abe-Suzuki, J. Karbanová, D. Corbeil, T. Itoh, Y. Kakeji and Y. Zen. (2018) SOX2-silenced squamous cell carcinoma: A highly malignant form of esophageal cancer with SOX2 promoter hypermethylation. Mod. Pathol. 31: 83-92 Medline


Bachor, T.P., J. Karbanová, E. Büttner, V. Bermúdez, M. Marquioni Ramella, P. Carmeliet, D. Corbeil§and A.M. Suburo§. (§ Corresponding authors).Early ciliary and prominin-1 dysfunctions precede neurogenesis impairment in a mouse model of type-2 diabetes. Neurobiol. Dis.108: 13-28 Medline

Lorico, A., G. Rappa, D. Corbeil, T.T. Le. Observation-driven inquiry: Raman spectroscopic imaging illuminates cancer lipid metabolism. Stem Cell Investig. 4.42Medline

Bobinger, T., L. May, H. Lücking, S.P. Kloska, P. Burkardt, P. Spitzer, J.M. Maler, D. Corbeil and H.B. Huttner. (2017) CD133-positive membrane particles in cerebrospinal fluid of patients with inflammatory and degenerative neurological diseases. Front. Cell. Neurosci.11: 1-9 Medline

Rappa, G., M.F. Santos, T.M. Green, J. Karbanová, J. Hassler, Y. Bai, S.H. Barsky, D. Corbeil§ and Aurelio Lorico. (§ Corresponding author). (2017)Nuclear transport of cancer extracellular vesicle-derived biomaterials through nuclear envelope invagination-associated late endosomes. Oncotarget8: 14443-14461.Medline

Sakai, Y., S.-M. Hong, S. An,J.Y. Kim, D. Corbeil, J.Karbanová, K. Otani, K. Fujikura, K.B. Song, S.C. Kim,M. Akita, Y. Nanno, H. Toyama, T. Fukumoto, Y. Ku, T. Hirose, T. Itoh and Y. Zen. CD133 expression in well-differentiated pancreatic neuroendocrine tumors: A potential predictor of progressive clinical courses. Hum Pathol61: 148-157>Medline


Thamm, K., S. Graupner, C. Werner, W.B. Huttner and D. Corbeil. (2016) Monoclonal Antibodies 13A4 and AC133 Do Not Recognize the Canine Ortholog of Mouse and Human Stem Cell Antigen Prominin-1 (CD133). PLoS One 11: e0164079 Medline

Fargeas, C.A., and D. Corbeil. (2016) Comments on the “Prognostic Impact and Clinicopathological Correlation of CD133 and ALDH1 Expression in Invasive Breast Cancer” and the “Commentary by Antonio Ieni and Giovanni Tuccari” J Breast Cancer 9: 336-338 Medline

Santos-Ferreira, T., M. Völkner, O. Borsch, J. Haas, P. Cimalla, P. Vasudevan, P. Carmeliet, D. Corbeil, S. Michalakis, E. Koch, M.O. Karl andM. Ader. (2016) Author Response: Possibility of cytoplasmic transportation between donor-host cell following photoreceptor transplantation.Invest. Ophthalmol. Vis. Sci. 57: 5336-5337Medline

Reichert, D., J. Scheinpflug, J. Karbanová, D. Freund, M. BornhäuserD. Corbeil. Tunnelling nanotubes mediate the transfer of stem cell marker CD133 between hematopoietic progenitor cells. Exp Hematol. 44:1092-1112Medline Front Cover

Niehage, C., J. Karbanová, C. Steenblock, D. Corbeil* and B. Hoflack*. (*Equal contribution) (2016) Cell surface proteome of dental pulp stem cells identified by label-free mass spectrometry. PloS One11: e0159824 Medline

Santos-Ferreira, T., M. Völkner, O. Borsch, J. Haas, P. Cimalla, P. Vasudevan, P. Carmeliet, D. Corbeil, S. Michalakis, E. Koch, M.O. Karland M. Ader. Stem cell-derived photoreceptor transplants differentially integrate into mouse models of cone-rod dystrophy. Invest. Ophthalmol. Vis. Sci. 57: 3509-3520 Medline


Reichert, D., J. Friedrichs, S. Ritter, T. Käubler, C. Werner, M. Bornhäuser and D. Corbeil. Phenotypic, morphological and adhesive differences of human hematopoietic progenitor cells cultured on murine versus human mesenchymal stromal Cells. Sci Rep. 5: 15680 Medline

Gouveia de Andrade, A.V., G. Bertolino, J. Riewaldt, K. Bieback, J. Karbanová, M. Odendahl, M. Bornhäuser, M. Schmitz, D. Corbeil, and T. Tonn.Extracellular vesicles secreted by bone marrow- and adipose tissue-derived mesenchymal stromal cells fail to suppress lymphocyte proliferation. Stem Cells Dev. 24: 1374-1376 Medline

Fargeas, C.A., E. Büttner, and D. Corbeil. Commentary: "Prom1 function in development, intestinal inflammation, and intestinal tumorigenesis" Front Oncol. 5: 1-4 Medline

Jászai, J., D. Corbeil§and C.A. Fargeas.(§ corresponding author) Comprehensive overview of CD133 biology in neural tissues across species. In Neural surface antigens – from basic biology towards biomedical applications. Edited by J. Pruszak. Elsevier Company. Chapter 10: 113-129

Rappa, G., T.M. Green, J. Karbanová, D. Corbeil and A. Lorico. Tetraspanin CD9 Determines Invasiveness and Tumorigenicity of Human Breast Cancer Cells. Oncotarget 6: 7970-7991Medline

Rappa, G., C.A. Fargeas, T.T. Le, D. Corbeiland A. Lorico.An Intriguing Relationship between Lipid Droplets, Cholesterol-Binding Protein CD133 and Wnt/b-catenin Signaling Pathway in Carcinogenesis. Stem Cells 33: 1366-1370 Medline


Karbanová, J., J. Laco, A-M.  Marzseco, P. Janich, M. Voborníková, J. Mokrý, C.A. Fargeas, W.B. Huttner and D. Corbeil. Human prominin-1 (CD133) is detected in both neoplastic and non-neoplastic salivary gland diseases and released into saliva in a ubiquitinated form. PLoS One 9: e98927 Medline

Corbeil, D., C.A. Fargeas and J. Jászai. CD133 might be a pan marker of epithelial cells with dedifferentiation capacity. Proc. Natl. Acad. Sci. USA  111: E1451-1452  Medline>


Jászai, J., S. Graupner, E.M. Tanaka, R.H.W. Funk, W.B. Huttner, M. Brand and D. Corbeil. Spatial distribution of prominin-1 (CD133)–positive cells within germinative zones of the vertebrate brains. PLoS One, 8: e63457 Medline

Arndt K., T. Grinenko, N. Mende, D. Reichert, M. Portz, T. Ripich, P. Carmeliet, D. Corbeil and C. Waskow. CD133 is a modifier of hematopoietic progenitor frequencies but is dispensable for the maintenance of mouse hematopoietic stem cells. Proc. Natl. Acad. Sci. USA  110: 5582-5587 Medline

Walker, T.L., A. Wierick, A.M. Sykes, B. Waldau, D. Corbeil, P. Carmeliet and G. Kempermann. Prominin-1 allows prospective isolation of neural stem cells from the adult murine hippocampus. J Neurosci. 33: 3010-3024 Medline

Corbeil, D., J. Karbanová, C.A. Fargeas and J. Jászai. Prominin-1 (CD133): Molecular and cellular features across species. In Prominin-1 (CD133): New Insights on Stem & Cancer Stem Cell Biology. Edited by D. Corbeil. Springer Company. Adv Exp Med Biol. 777: 3-24 Medline

Gurudev, N., M. Florek, D. Corbeil and E. Knust. Prominent role of prominin in the retina. In Prominin-1 (CD133): New Insights on Stem & Cancer Stem Cell Biology. Edited by D. Corbeil. Springer Company. Adv Exp Med Biol. 777: 55-71 Medline

Bernstein, P., I. Sperling, D. Corbeil, U. Hempel, S. Fickert. Progenitor cells from cartilage: no osteoarthritis-grade-specific differences in stem cell marker expression. Biotechnol. Prog. 29: 206-12 Medline

Rappa, G., J. Mercapide, F. Anzanello, T.T. Le, M.G. Johlfs, R.R. Fiscus, M. Wilsch-Bräuninger, D. Corbeil and A. Lorico. Wnt Interaction and extracellular release of prominin-1/CD133 in human malignant melanoma cells. Exp Cell Res. 319: 810-819 Medline

Wittig, D., J. Jászai, D. Corbeil and R.H.W. Funk. Immunohistochemical localization and characterization of putative mesenchymal stem cell markers in the retinal capillary network of rodents. Cells Tiss Org.  2: 344-359 Medline

Grosse-Gehling, P., C.A. Fargeas, C. Dittfeld, Y. Garbe, M.R. Alison, D. Corbeil and L.A. Kunz-Schughart. CD133 as a biomarker for putative cancer stem cells in solid tumours: limitations, problems and challenges. J Pathol. 229: 355-78 Medline


Eberle, D., T. Kurth, T. Santos-Ferreira, J. Wilson, D. Corbeil and M. Ader. Outer segment formation of transplanted photoreceptor precursor cells. PLoS One 7: e46305 Medline

Fonseca, A.-V., D. Reichert and D. Corbeil. Migration of stem cells – Role of the RhoA / ROCK I pathway (Method). Stem Cells and Cancer Stem Cells 6: 319-331 Medline

Huttner, H.B., D. Corbeil, C. Thirmeyer, R. Coras, M. Köhrmann, C. Mauer, J.B. Kuramatsu, S. Kloska, A. Doerfler, D. Weigel, J, Klucken, J, Winkler, E, Pauli, S. Schwab, H.M. Hamer and B.S. Kasper. Increased membrane shedding - indicated by an elevation of CD133-enriched membrane particles - into the CSF in partial epilepsy. Epilepsy Res. 99: 101-6 Medline


Ettinger, A.W., M. Wilsch-Bräuninger, A.-M. Marzesco, M. Bickle, A. Lohmann, Z. Maliga, J. Karbanová, D. Corbeil, A.A. Hyman and W.B. Huttner. Proliferating versus differentiating stem and cancer cells exhibit distinct midbody release behavior. Nature Commun. 2: 503 (1-12) Medline

Fargeas, C.A., J. Karbanová, J. Jászai and D. Corbeil. CD133 and membrane microdomains ? Old facets for future hypotheses. World J Gastroenterol. 17: 4149-4152 Medline

Lathia, J.D., M. Hitomi, J. Gallagher, S.P. Gadani, J. Adkins, A. Vasanji, L. Liu, C.E. Eyler, J.M. Heddleston, Q. Wu, S. Minhas, A. Soeda, D.J. Hoeppner, R. Ravin, R.D.G. McKay, R.E. McLendon, D. Corbeil, A. Chenn, A.B. Hjelmeland, D.M. Park and J.N. Rich. Distribution of CD133 reveals glioma stem cells self-renew through symmetric and asymmetric cell divisions. Cell Death Dis. 2: e200 Medline

Eberle, D., S. Schubert, K. Postel, D. Corbeil, M. Ader. Increased integration of transplanted CD73-positive photoreceptor precursors into adult mouse retina. Invest. Ophthalmol. Vis. Sci. 52: 6462-6471  Medline

Niehage, C., C. Steenblock, T. Pursche, M. Bornhäuser, D. Corbeil and B. Hoflack. The cell surface proteome of human mesenchymal stromal cells. PloS One. 6: e20399 Medline

Bauer, N., M. Wilsch-Bräuninger, J. Karbanová, A.-V. Fonseca, D. Strauss, D. Freund, C. Thiele, W.B. Huttner, M. Bornhäuser and D. Corbeil.  Hematopoietic stem cell differentiation promotes the release of prominin-1/CD133?containing membrane vesicles ? A role of the endocytic-exocytic pathway. EMBO Mol. Med. 3: 398-409 Medline

Zen, C., Y. Zen, R.R. Mitry, D. Corbeil, J. Karbanová, J. O?Grady, J. Karani, P. Kane, N. Heaton, B.C. Portmann, and A. Quaglia. Mixed phenotype hepatocellular carcinoma after trans-arterial chemoembolization (TACE) and liver transplantation. Liver Transplant. 17: 943-954  Medline

Jászai, J., C.A. Fargeas, S. Graupner, E.M. Tanaka, M. Brand, W.B. Huttner and D. Corbeil. Distinct and conserved prominin-1/CD133-positive retinal cell populations identified across species. PloS One. 6: e17590 Medline

Fonseca, A.-V. and D. Corbeil. The hematopoietic stem cell polarization and migration: A dynamic link between RhoA signaling pathway, microtubule network and ganglioside-based membrane microdomains. Commun Integr Biol. 4: 201-204 Front Cover Medline 

Fickert, S., U. Schröter-Bobsin, A.F. Groß, U. Hempel, C. Wojciechowski, C. Rentsch, D. Corbeil and K.P. Günther. Human mesenchymal stem cell proliferation and osteogenic differentiation during long-term ex vivo cultivation is not age dependent. J. Bone Miner. Metab. 29: 224-235 Medline

Karbanová, J., T. Soukup, J. Suchánek, R. Pytlík, D. Corbeil and J. Mokrý. Characterization of dental pulp stem cells from impacted third molars cultured in low-serum containing medium. Cells Tissues Organs. 193: 344-365 Medline

Missol-Kolka, E., J. Karbanová, P. Janich, M. Haase, C.A. Fargeas, W.B. Huttner and D. Corbeil. Prominin-1 (CD133) is not restricted to stem cells located in the basal compartment of murine and human prostate. The Prostate. 71: 254-263 Medline


Fonseca, A.-V., D. Freund, M. Bornhäuser and D. Corbeil. Polarization and migration of hematopoietic stem and progenitor cells rely on RhoA/ROCK I pathway and an active reorganization of the microtubule network. J. Biol. Chem. 285: 31661-31671 Medline

Fietz, S.A., I. Kelava, J. Vogt, Wilsch-Bräuninger D. Stezel, J.L. Fish, D. Corbeil, Riehn, W. Distler, R. Nitsch and W.B. Huttner. Outer subventricular zone progenitors of human and ferret cerebral cortex retain epithelial properties and expand via integrin signaling.
Nature Neurosci.13: 690-699 Medline

Jászai, J., L.M. Farkas, C.A. Fargeas, P. Janich, M. Haase, W.B. Huttner and D. Corbeil. Prominin-2 is a novel marker of distal tubules and collecting ducts of the human and murine kidney. Histochem. Cell Biol. 133: 527-539 Medline

Jing, D., A.-V. Fonseca, N. Alakel, F.A. Fierro, K. Muller, M. Bornhäuser, G. Ehninger, D. Corbeil and R. Ordemann. Hematopoietic stem cells in coculture with mesenchymal stromal cells - modelling the niche compartments in-vitro. Haematologica. 95: 542-550  Medline

Hundsdoerfer, A.K., M. Sanetra, D. Corbeil and H. Stuckas. Eleven Hawkmoth Microsatellite Loci of Canary Island Hyles tithymali (Lepidoptera). Conservation Genet. Resour. 2: 241-244 Medline

Corbeil, D., A,-M. Marzesco, M. Wilsch-Bräuninger and W.B. Huttner. The intriguing links between prominin-1 (CD133), cholesterol-based membrane microdomains, remodeling of apical plasma membrane protrusions, extracellular membrane particles, and (neuro)epithelial cell differentiation. FEBS Lett. 584: 1659-1664 Medline

Freund, D., A.-V. Fonseca, P. Janich, M. Bornhäuser and D. Corbeil. Differential expression of biofunctional GM1 and GM3 gangliosides within the plastic-adherent multipotent mesenchymal stromal cell population. Cytotherapy. 12:131-142 Medline

Corbeil, D., A.-M. Marzesco, C.A. Fargeas, and W.B. Huttner. Prominin-1, a distinct cholesterol?binding membrane protein, and the organisation of the apical plasma membrane of epithelial cells. Subcell. Biochem. 51:399-423 Medline


 Gedye, C., J. Quirk, J. Browning, S. Svobodova, T. John, P Sluka, P.R. Dunbar, D. Corbeil, J., Cebon and I.D. Davis. Cancer/testis antigens can be immunological targets in clonogenic CD133+ melanoma cells. Cancer Immunol. Immunother. 58:1635-1646 Medline 

 Marzesco, A.-M., M. Wilsch-Bräuninger, V. Dubreuil, P. Janich, K. Langenfeld, C. Thiele, W.B. Huttner and D. Corbeil. Release of extracellular membrane vesicles from microvilli of epithelial cells is enhanced by depleting membrane cholesterol. FEBS Lett. 583:897-902 Medline 

 Zacchigna, S., H. Oh, M. Wilsch-Bräuninger, E. Missol-Kolka, M. J. Jászai, S. Jansen, N. Tanimoto, F. Tonagel, M. Seeliger, W.B. Huttner, D. Corbeil, M. Dewerchin, S. Vinckier, L. Moons and P. Carmeliet. Loss of cholesterol-binding protein prominin-1/CD133 causes disk dysmorphogenesis and photoreceptor degeneration. J. Neurosci. 29: 2297-2308 Medline 

 Bernstein, P., M. Dong, D. Corbeil, M. Gelinsky, K.-P. Günther and S. Fickert. Pellet culture elicits superior chondrogenic redifferentiation than alginate-based systems. Biotechnol. Prog. 25:1146-1152 

Corbeil, D., A. Joester, C.A. Fargeas, J. Jászai, J. Garwood, H.B. Werner and W.B. Huttner. (§ corresponding author). Expression of distinct splice variants of the stem cell marker Prominin-1 (CD133) in glial cells. Glia. 57:860-874 Medline 

Bernstein, P., M. Dong, S. Graupner, D. Corbeil, M. Gelinsky, K.-P. Günther and S. Fickert. Sox9 expression of alginate-encapsulated chondrocytes is stimulated by low cell density. J. Biomed. Mat. Res. Part A. 91:910-918 Medline 

Siebzehnrübl, F.A., I. Jesker, D. Müller, R. Buslei, R. Coras, E. Hahnen, H.B. Huttner, D. Corbeil, J. Kaesbauer, T. Appl, S. von Hörsten and I. Blümcke. Spontaneous in vitro transformation of adult neural precursors into stem-like cancer cells. Brain Pathol. 19:399-408 Medline


 Karbanová, J., E. Missol-Kolka, A.-V. Fonseca, C. Lorra, P. Janich, H. Hollerová, J. Jászai, J. Ehrmann, Z. Kolá?, C. Liebers, S. Arl, D. ?ubrtová, D. Freund, J. Mokrý, W.B. Huttner and D. Corbeil. The stem cell marker CD133 (Prominin-1) is expressed in various human glandular epithelia. J Histochem Cytochem. 56:977-993 Medline Suppl. Mat.

Yang, Z., Y. Chen, C.Lillo, J. Chien, M. Michaelides, M. Klein K.A. Howes, Y. Li, Y. Kaminoh, H. Chen, C. Zhao, Y. Chen, Y.T. Al-Sheikh, G. Karan, D. Corbeil, P. Escher, S. Kamaya, C. Li, S. Johnson, J.M. Frederick, Y. Zhao, C. Wang, D.J. Cameron, W.B. Huttner, D.F. Schorderet, F.L. Munier, A.T. Moore, D.G. Birch, W. Baehr, D. M. Hunt, D.S. Williams and K. Zhang. Mutant prominin-1 found in patients with macular degeneration disrupts photoreceptor disk morphogenesis in mice. J Clin Invest. 118:2908-2916 Medline 

 Jászai, J., C.A. Fargeas, M. Haase, L.M. Farkas, W.B. Huttner and D. Corbeil. Robust expression of Prominin-2 all along the adult male reproductive system and urinary bladder. Histochem Cell Biol. 130:749-759 Medline  

Fonseca, A.-V., N. Bauer and D. Corbeil. The stem cell marker CD133 meets the endosomal compartment ? New insights into the cell division of hematopoietic stem cells. Blood Cells Mol Dis. 41: 194-195 Medline Suppl. Mat. 

 Huttner, H.B., P. Janich, M. Köhrmann, J. Jászai, F. Siebzehnrubl, I. Blümcke, M. Suttorp, M. Gahr, D. Kuhnt, C. Nimsky, D. Krex, G. Schackert, K. Löwenbrück, H. Reichmann, E. Jüttler, W. Hacke, P. D. Schellinger, S. Schwab, M. Wilsch-Bräuninger, A.-M. Marzesco, and D. Corbeil. The stem cell marker prominin-1/CD133 on membrane particles in human cerebrospinal fluid offers novel approaches for studying CNS disease. Stem Cells. 26: 698-705 Medline  

Bauer, N., A.-V. Fonseca, M. Florek, D. Freund, J. Jászai, M. Bornhäuser, C.A. Fargeas and D. Corbeil. New insights into the cell biology of hematopoietic progenitors by studying prominin-1 (CD133). Cells Tiss Org. 188: 127-138 Medline Front Cover

 Lardon, J., D. Corbeil, W.B. Huttner, Z. Ling and L. Bouwens. Stem cell marker prominin 1/AC133 is expressed in duct cells of the adult human pancreas. Pancreas. 36: e1-e6 Medline


 Jászai, J., C.A. Fargeas, M. Florek, W.B. Huttner and D. Corbeil. Focus on Molecules: Prominin-1 (CD133). Exp. Eye Res. 85: 585-586 Medline

Jászai, J., P. Janich, L.M. Farkas, C.A. Fargeas, W.B. Huttner and D. Corbeil. Differential expression of Prominin-1 (CD133) and Prominin-2 in major cephalic exocrine glands of adult mice. Histochem Cell Biol. 128: 409-419 Medline 

Janich, P. and D. Corbeil. GM1 and GM3 gangliosides highlight distinct lipid microdomains within the apical domain of epithelial cells. FEBS Lett. 581: 1783-1787 Medline

Fargeas, C.A., W.B. Huttner and D. Corbeil. Nomenclature of prominin-1 (CD133) splice variants â?? An update. Tissue Antigens. 69: 602-606 Medline

 Rammelt, S., D. Corbeil, S. Manthey, H, Zwipp and U. Hanisch. Immunohistochemical in-situ characterization of orthopaedic implants on PMMA embedded cutting and grinding sections. J. Biomed. Mat. Res. Part A. 83: 313-322 Medline

Dubreuil, D., A.-M. Marzesco, D. Corbeil, W.B. Huttner and M. Wilsch-Bräuninger. Midbody and primary cilium of neural progenitors release extracellular membrane particles enriched in the stem cell marker prominin-1. J. Cell Biol. 176: 483-495 Medline

Sicard, F., M. Ehrart-Bornstein, D. Corbeil, S. Sperber, A.W. Krug, C.G. Ziegler, V. Rettori, S.M. McCann and S.R. Bornstein. Growth factors, DHEA and DHEAS regulate chromaffin cell proliferation in an age-dependent manner. Proc. Natl. Acad. Sci. USA. 104: 2007-2012 Medline

Florek, M., N. Bauer, P. Janich, M. Wilsch-Braeuninger, C.A. Fargeas, A.-M. Marzesco, C. Thiele, G. Ehninger, W.B. Huttner and D. Corbeil. Prominin-2 is a cholesterol-binding protein associated with apical and basolateral plasmalemmal protrusions in polarized epithelial cells and released into the urine. Cell Tissue Res. 328: 31-47 MedlineFront Cover 


 Freund, D., N. Bauer S. Boxberger, B. Jørgensen, S. Feldmann, G. Ehninger, C. Werner, M. Bornhäuser, J. Oswald and D. Corbeil.  Polarization of human hematopoietic progenitors during contact with multipotent mesenchymal stromal cells ? effects on proliferation and clonogenicity. Stem Cells Dev. 15: 815-829 Medline Front Cover 
 Freund, D., J. Oswald, S. Feldmann, G. Ehninger, D. Corbeil and M. Bornhäuser. Comparative analysis of proliferative potential and clonogenicity of MACS-immunomagnetic isolated CD34+ and CD133+ blood stem cells derived from a single donor. Cell Prolif. 39: 325-332 Medline
 Fargeas, C.A., A.-V. Fonseca, W.B. Huttner and D. Corbeil. Prominin-1 (CD133) ? from progenitor cells to human diseases. Future Lipidology. 2: 213-225 Medline


 Marzesco, A-M., P. Janich, M. Wilsch-Bräuninger, V. Dubreuil, K. Langenfeld, D. Corbeil and W.B. Huttner. Release of extracellular membrane particles carrying the stem cell marker prominin-1 (CD133) from neural progenitors and other epithelial cells. J. Cell Sci.118: 2849-2858 Medline 
 Lee, A., J.D. Kessler, T.-A. Read, C. Kaiser, D. Corbeil, W.B. Huttner, J.E. Johnson, R.J. Wechsler-Reya. Isolation of neural stem cells from postnatal cerebellum. Nat. Neurosci. 8: 715-719 Medline 
 Kania, G., D. Corbeil, J. Fuchs, K.V. Tarasov, P. Blyszczuk, W.B. Huttner, K.R. Boheler and A.M. Wobus. The somatic stem cell marker prominin-1/CD133 is expressed in embryonic stem cell-derived progenitors. Stem Cells. 23: 791-804 Medline 
 Corbeil, D., C. Thiele and W. B. Huttner. Tyrosine sulfation (Unit 14.7). Current Protocols in Protein Science. Edited by J. Colligan, B.M. Dunn. D.W. Speicher and P.T. Wingfield. Wiley & Sons Press. Suppl. 39:14.7.1-14.7.14
 Florek, M., M. Haase, A.-M. Marzesco, D. Freund, G. Ehninger, W. B. Huttner and D. Corbeil. Prominin-1/CD133, a neural and hematopoietic stem cell marker, is expressed in adult human differentiated cells and certain types of kidney cancer. Cell Tissue Res. 319: 15-26 Medline 
 Bornhäuser, M., L. Eger, U. Oelschlaegel, S. Auffermann-Gretzinger, A. Kiani, J. Schetelig, T. Illmer, M. Schaich, D. Corbeil, C. Thiede and G. Ehninger. Rapid reconstitution of dendritic cells after allogeneic transplatation of CD133+ selected hematopoietic stem cells. Leukemia. 19: 161-165 


 Fargeas, C.A., A. Joester, E. Missol-Kolka, A. Hellwig, W.B. Huttner and D. Corbeil. Identification of novel Prominin-1/CD133 splice variants with alternative C-termini and their expression in epididymis and testis. J. Cell Sci. 117: 4301-4311 Medline 
 Corbeil, D., and W.B. Huttner. Tyrosine Sulfation. Encyclopedia Biol. Chem. Edited by W. Lennarz and M.D. Lane. Elsevier, Oxford. 4: 294-297
 Giebel, G., D. Corbeil, J. Beckmann, J. Höhn, D. Freund, K. Giesen, G. Kögler and P. Wernet. Segregation of lipid raft markers including CD133 in polarized human hematopoietic stem and progenitor cells. Blood. 104: 2332-2338 Medline 
 Kosodo, Y., K. Röper, W. Haubensak, A.-M. Marzesco, D. Corbeil and W.B. Huttner. Asymmetric distribution of the apical plasma membrane during neurogenic divisions of mammalian neuroepithelial cells. EMBO J. 23: 2314-2324 Medline



 Fargeas, C.A., D. Corbeil and W.B. Huttner. AC133 antigen, CD133, prominin-1, prominin-2, Etc.: Prominin family gene products in need of a rational nomenclature. Stem Cells. 21: 506-508 Medline 
 Fargeas, C.A., M. Florek, W.B. Huttner and D. Corbeil. Characterization of Prominin-2, a new member of the prominin family of pentaspan membrane glycoproteins. J. Biol. Chem. 278: 8586-8596 Medline 


 Rustom, A., M. Bajohrs, C. Kaether, P. Keller, D. Toomre, D. Corbeil and H.-H. Gerdes. Selective delivery of secretory cargo in Golgi-derived carriers of nonepithelial cells. Traffic. 3: 279-288


 Corbeil, D., C. A. Fargeas and W.B. Huttner. Rat prominin, like its mouse and human orthologues, is a pentaspan membrane glycoprotein. Biochem. Biophys. Res. Commun. 285: 939-944 Medline
 Corbeil, D., K. Röper, C.A. Fargeas, A. Joester and W.B. Huttner. Prominin: A story of cholesterol, plasma membrane protrusions and human pathology. Traffic. 2: 82-91 Medline 


 Röper*, K., D. Corbeil* and W.B. Huttner. (*joint first authors). Retention of prominin in microvilli reveals distinct cholesterol-based lipid microdomain within the apical membrane of epithelial cells. Nature Cell Biol. 2: 582-592 Medline 
 Corbeil, D., K. Röper, A. Hellwig, M. Tavian, S. Miraglia, S.M. Watt, P.J. Simmons, B. Peault, D.W. Buck and W.B. Huttner. The human AC133 hematopoietic stem cell antigen is also expressed in epithelial cells and targeted in membrane protrusions. J. Biol. Chem. 275: 5512-5520 Medline 
 Röper*, K., D. Corbeil* and W.B. Huttner. (*joint first authors). Distinct lipid microdomains within the apical plasma membrane of polarized epithelial cells. A potential role of the lipid bilayer in the retention of prominin in microvilli. In Protein, Lipid and Membrane Traffic: Pathway and Targeting. Edited by Jos A.F. Op den Kamp. IOS Press. NATO Science Series. Vol. 322: 73-84
 Maw, M.A., D. Corbeil, J. Koch, A. Hellwig, J.C. Wilson-Wheeler, R.J. Bridges, G. Kumaramanickavel, S. John, D. Nacarrow, K. Röper, A. Weigmann, W.B. Huttner and M.J. Denton. A frameshift mutation in prominin (mouse)-like 1 causes human retinal degeneration. Hum. Mol. Genet. 9: 27-34 Medline 


 Corbeil, D., K. Röper, M.J. Hannah, A. Hellwig and W.B. Huttner. Selective localization of the polytopic membrane protein prominin in microvilli of epithelial cells- A combination of apical sorting and retention in plasma membrane protrusions. J. Cell Sci. 112: 1023-1033 Medline 


 Beißwanger, R., D. Corbeil, C. Vannier, C. Thiele, U. Dohrmann, R. Kellner, K. Ashman, C. Niehrs and W.B. Huttner. Existence of distinct tyrosylprotein sulfotransferase genes: Molecular characterization of tyrosylprotein sulfotransferase-2. Proc. Natl. Acad. Sci. USA. 95: 11134-11139
 Corbeil, D., K. Röper, A. Weigmann and W.B. Huttner. AC133 hematopoietic stem cell antigen: human homologue of mouse kidney prominin, or distinct member of a novel protein family? Blood. 91: 2625-2626 Medline


 Weigmann* A., D. Corbeil*, A. Hellwig and W.B. Huttner. (*joint first authors). Prominin, a novel microvilli-specific polytopic membrane protein of the apical surface of epithelial cells, is targeted to plasmalemmal protrusions of nonepithelial cells. Proc. Natl. Acad. Sci. USA. 94: 12425-12430 Medline 


 Huttner, W.B., M. Ohashi, R.H. Kehlenbach, F.A. Barr, R. Bauerfeind, O. Bräunling, D. Corbeil, M. Hannah, H.A. Pasolli, A. Schmidt, A.A. Schmidt, C. Thiele, Y. Wang, A. Krömer and H.-H. Gerdes. Biogenesis of neurosecretory vesicles. Cold Spring Harb. Symp. Quant. Biol. 60: 315-327
 Milhiet, P.-E., S. Chevallier, D. Corbeil, N.G. Seidah, P. Crine and G. Boileau. Proteolytic processing of the alpha subunit of rat endopeptidase-24.18 by furin. Biochem. J. 309: 683-688


 Milhiet, P.-E., D. Corbeil, V. Simon, A.J. Kenny, P. Crine and G. Boileau. Expression of rat endopeptidase-24.18 in COS-1 cells: membrane topology and activity. Biochem. J. 300: 37-43


 Corbeil, D., P.-E. Milhiet, V. Simon, J. Ingram, A.J. Kenny, G. Boileau and P. Crine. Rat endopeptidase-24.18 alpha subunit is secreted into the culture medium as a zymogen when expressed by COS-1 cells. FEBS Lett. 335: 361-366
 Crine, P., F. Jalal, C. Lanctôt, D. Corbeil, G. Waksman, G. Lemay, K. Withers and G. Boileau. Targeting of neutral endopeptidase-24.11 in polarized cells. Biochem. Soc. Trans. 21: 668-672


 Jalal, F., C. Jumarie, W. Bawab, D. Corbeil, C. Malo, A. Berteloot and P. Crine. Polarized distribution of neutral endopeptidase 24.11 at the cell surface of cultured human intestinal epithelial Caco-2 cells. Biochem. J. 288: 945-951
 Corbeil, D., F. Gaudoux, S. Wainwright, J. Ingram, A. J. Kenny, G. Boileau and P. Crine. Molecular cloning of the alpha-subunit of rat endopeptidase-24.18 (endopeptidase-2) and co-localization with endopeptidase-24.11 in rat kidney by in situ hybridization. FEBS Lett. 309: 203-208
 Corbeil, D., G. Boileau, G. Lemay and P. Crine. Expression and polarized apical secretion in Madin-Darby canine kidney cells of a recombinant soluble form of neutral endopeptidase lacking the cytosolic and transmembrane domains. J. Biol. Chem. 267: 2798-2801

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