Jun Fu
1987-1991: study at Northwest Forestry College, China (B. Agri.)
1991-1998: work at Shaanxi Academy of Forestry, China
1998-2001: study at TU Dresden (MSc. Forestry)
2002-2004: work at Gene Bridges GmbH, Dresden
since 2004: work at BIOTEC, TU Dresden
Research interests
Research and development of recombineering
Gene targeting and BAC transgenesis
PKS/NRPS gene clusters engineering and heterologous expression
Publications
Wojtasz, L., Cloutier, J., Baumann, M., Daniel, K., Varga, J., Fu, J., Anastassiadis, K., Stewart, A. F., Remenyi, A., Turner, J. and Toth, A. (2012) “Meiotic DNA double strand breaks and chromosome asynapsis in mouse are monitored by distinct HORMAD2-independent and -dependent mechanisms.” Genes Dev 26:958-973.
Fu, J., Bian, X., Hu, S., Wang, H., Huang, F., Seibert, P., Plaza, A., Xia, L., Müller, R., Stewart, A. F. and Zhang, Y. (2012) "Enhanced direct DNA cloning by full length RecE and its application to bioprospecting for secondary metabolites.” Nat Biotec DOI:10.1038/nbt.2183.
Bird, A. W., Erler, A., Fu, J., Hériché, J. K., Maresca, M., Zhang, Y., Hyman, A. A. and Stewart, A. F. (2011). "High efficiency counterselection recombineering for site-directed mutagenesis in bacterial artificial chromosomes." Nat Methods 9:103-109.
Nedelkova, M., Maresca, M., Fu, J., Rostovskaya, M., Chenna, R., Thiede, C., Anastasiadis, K., Sarov, M. and Stewart, A. F. (2011). "Targeted isolation of cloned genomic regions by recombineering for haplotype phasing and isogenic targeting." Nucleic Acids Res 39:e137.
Skarnes, W. C., Rosen, B., West, A. P., Koutsourakis, M., Bushell, W., Iyer, V., Cox, R., Jackson, D., Severin, J., Biggs, P., Thomas, M., Mujica, A., Harrow, J., Fu, J., Nefedov, M., de Jong, P., Stewart, A. F. and Bradley, A. (2011). "A conditional knockout resource for genome-wide analysis of mouse gene function." Nature 474:62-263.
Daniel, K., Lange, J., Hached, K., Fu, J., Anastassiadis, K., Roig, I., Cooke, H. J., Stewart, A. F., Wassmann, K., Jasin, M., Keeney, S. and Toth, A. (2011). "Meiotic homologue alignment and its quality surveillance are controlled by mouse HORMAD1." Nat Cell Biol 13:599-610.
Hofemeister, H., Ciotta, G., Fu, J., Seibert, P. M., Schulz, A., Maresca, M., Sarov, M., Anastassiadis, K. and Stewart, A. F. (2010). "Recombineering, transfection, Western, IP and ChIP methods for protein tagging via gene targeting or BAC transgenesis." Methods.
Ciotta, G., Hofemeister, H., Maresca, M., Fu, J., Sarov, M., Anastassiadis, K. and Stewart, A. F. (2010). "Recombineering BAC transgenes for protein tagging." Methods.
Fu, J., Teucher, M., Anastassiadis, K., Skarnes, W. and Stewart, A. F. (2010). "A Recombineering Pipeline to Make Conditional Targeting Constructs." Methods Enzymol 477C: 125-144.
Maresca, M., Erler, A., Fu, J., Friedrich, A., Zhang, Y. and Stewart, A. F. (2010). "Single-stranded heteroduplex intermediates in lambda Red homologous recombination." BMC Mol Biol 11: 54.
Kranz, A., Fu, J., Duerschke, K., Weidlich, S., Naumann, R., Stewart, A. F. and Anastassiadis, K. (2010). "An improved Flp deleter mouse in C57Bl/6 based on Flpo recombinase." Genesis.
Anastassiadis, K., Fu, J., Patsch, C., Hu, S., Weidlich, S., Duerschke, K., Buchholz, F., Edenhofer, F. and Stewart, A. F. (2009). "Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice." Dis Model Mech 2: 508-15.
Fu, J., Wenzel, S. C., Perlova, O., Wang, J., Gross, F., Tang, Z., Yin, Y., Stewart, A. F., Muller, R. and Zhang, Y. (2008). "Efficient transfer of two large secondary metabolite pathway gene clusters into heterologous hosts by transposition." Nucleic Acids Res 36: e113.
Erler, A., Maresca, M., Fu, J. and Stewart, A. F. (2006). "Recombineering reagents for improved inducible expression and selection marker re-use in Schizosaccharomyces pombe." Yeast 23: 813-23.
Gross, F., Ring, M. W., Perlova, O., Fu, J., Schneider, S., Gerth, K., Kuhlmann, S., Stewart, A. F., Zhang, Y. and Muller, R. (2006). "Metabolic engineering of Pseudomonas putida for methylmalonyl-CoA biosynthesis to enable complex heterologous secondary metabolite formation." Chem Biol 13: 1253-64.
Perlova, O., Fu, J., Kuhlmann, S., Krug, D., Stewart, A. F., Zhang, Y. and Muller, R. (2006). "Reconstitution of the myxothiazol biosynthetic gene cluster by Red/ET recombination and heterologous expression in Myxococcus xanthus." Appl Environ Microbiol 72: 7485-94.
Wang, J., Sarov, M., Rientjes, J., Fu, J., Hollak, H., Kranz, H., Xie, W., Stewart, A. F. and Zhang, Y. (2006). "An improved recombineering approach by adding RecA to lambda Red recombination." Mol Biotechnol 32: 43-53.
Wenzel, S. C., Gross, F., Zhang, Y., Fu, J., Stewart, A. F. and Muller, R. (2005). "Heterologous expression of a myxobacterial natural products assembly line in pseudomonads via red/ET recombineering." Chem Biol 12: 349-56.
Patents
FU, Jun; ZHANG, Youming; STEWART, Francis. “Direct cloning of DNA fragment from genomic DNA without library construction and screening” PCT/IB2011/052549
ZHANG, Youming; FU, Jun; BIAN, Xiaoying; STEWART, Francis; ZIPS, Daniel; MÜLLER, Rolf “Heterologous expression of PKS/NRPS gene cluster and targeted anticancer drug delivery.” PCT/IB2010/055923
MARESCA, Marcello; ERLER, Axel, Steffen; FU, Jun; SEIBERT, Philipp, Martin; STEWART, Adrian, Francis; ZHANG, Youming "Method of nucleic acid recombination" PCT/IB2009/000488
ZHANG, Youming; MÜLLER, Rolf; STEWART, Francis; GROSS, Frank; WENZEL,Silke, C.; FU, Jun "Methods for heterologous expression of secondary metabolites" PCT/IB2005/003650; EP1809749 (2007); US20090298117 (2009)
