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Piconewton-sensitive biosensors to investigate adhesion mechanics in cells

CMCB Life Sciences Seminar

Date:24.10.2019, 16:00 - 17:00
Speaker: Prof. Carsten Grashoff, University of Münster
Location: CRTD, ground floor, auditorium left
Host: Dr. Gokul Kesavan (CRTD)

To investigate the molecular mechanisms underlying cell adhesion mechanics, we developed a set of single-molecule‒calibrated biosensors that are sensitive to physiologically relevant forces in the low piconewton range. These biosensors are genetically encoded and can be utilized to determine molecular forces acting across individual molecules in cells. In my seminar, I will describe how their application to the focal adhesion protein talin and the desmosomal molecule desmoplakin revealed fundamental differences in how distinct adhesion molecules modulate intracellular force transduction.

5 most important publications
1. Price AJ, Cost AL, Ungewiβ H, Waschke J, Dunn AR, and Grashoff C. 2018. Nat Commun 11; 9:5284.
2. Ringer, P. Weißl, A., Cost, A.L., Freikamp, A., Sabass, B., Mehlich, A., Tramier, M., Rief, M. and Grashoff, C. 2017. Nat Methods 14: 1090-1096.
3. Austen KA, Ringer P, Mehlich A, Chrostek-Grashoff A, Kluger C, Klingner C, Sabass B, Zent R, Rief M, and Grashoff C. 2015. Nat Cell Biol 17: 1597-1606.
4. Hoffman BD, Grashoff C, and Schwartz MA. 2011. Nature 475(7356): 316-323.
5. Grashoff C, Hoffman BD, Brenner MD, Zhou R, Parsons M, Yang MT, McLean MA, Sligar SG, Chen CS, Ha T, and Schwartz MA. 2010. Nature 466(7303): 263-266.

Everybody is very welcome!

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