Examplepictures of DNA-Structures

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October 01 - October 31

7 entries found

Microfluidi..
Start date: October 07
11:00 am 12:00 pm

Description: 

Please contact the host (jochen.guck@biotec.tu-dresden.de) if you would like to talk to the speaker. Everybody is welcome!

Abstract:
Cell deformability (i.e., the ability to change shape under an applied force) is a promising physical marker indicative of underlying structural changes associated with various disease processes and changes in cell state.  We are combining precision microfluidic control of cells with automated high-speed image analysis for high-throughput cell classification based on intrinsic biomechanical properties.  I will first discuss general strategies we are developing to passively manipulate particles and fluids using simple geometric modifications within microchannels.  Our approaches make use of fluid inertia, generally neglected in microfluidic systems, to create well-defined directional forces and fluid deformations that can be combined in a sequential and hierarchical manner to program complex particle and fluid motions. Low complexity modular components to manipulate cells, particles, and fluid streams in which inertial fluid physics is abstracted from the designer has the capability transform biological, chemical, and materials automation in a similar fashion to how modular control of electrons and abstraction of semiconductor physics transformed computation.  We apply these fundamental techniques to position cells for high-speed fluid-based deformation and optical analysis. The “deformability cytometer” instrument shows promise in identifying cancer cells, activated white blood cells, and stem cells in mixed populations – without labels - for a variety of clinical and regenerative medicine applications.

Recent Publications:
Tse HTK, Gossett DR, Moon YS, Masaeli M, Sohsman M, Ying Y, Mislick K, Adams RP, Rao J, Di Carlo D. Quantitative Diagnosis of Malignant Pleural Effusions by Single-Cell Mechanophenotyping. Science Translational Medicine (2013). doi: 10.1126/scitranslmed.300655

Sollier E, Go DE, Che J, Daniel Gossett, Sean O'Byrne, Weaver WM, Kummer N, Rettig M, Goldman J, Nickols N, McCloskey S, Kulkarni R and Di Carlo D. Size-Selective Collection of Circulating Tumor Cells using Vortex Technology. Lab on a Chip (2013). doi: 10.1039/C3LC50689D

Amini H, Sollier E, Masaeli M, Xie Y, Ganapathysubramanian B, Stone HA, and Di Carlo D. Engineering fluid flow using sequenced microstructures. Nature Communications (2013). doi:10.1038/ncomms2841

Tseng, P, Judy JW, Di Carlo D. Magnetic nanoparticle-mediated massively parallel mechanical modulation of single-cell behavior. Nature Methods (2012). doi:10.1038/nmeth.2210

Gossett DR, Tse HTK, Lee SA, Ying Y, Lindgren AG, Yang OO, Rao J, Clark AT, Di Carlo D. Hydrodynamic stretching of single cells for large population mechanical phenotyping. PNAS (2012) doi: 10.1073/pnas.1200107109.

Cytometry A..
Start date: October 15
End date:  - October 17
08:00 am 04:00 pm

Description: 

The DGfZ 2014 will take place at the Center for Regenerative Therapies Dresden (CRTD ) in Dresden from October 15th – 17th 2014 and we are happy to welcome you again.

More than 20 exhibitors and 200 participants is the result of last years DGfZ meeting at the Center for Regenerative Therapies Dresden (CRTD ) .” Cytometry Across Disciplines ” was the topic, which has attracted considerable interest. The positive response from exhibitors , guest speakers and participants from over 10 countries, is a confirmation for the organizers , Prof. Dr. Leoni Kunz- Schughart (National Centre for Radiation Research in Oncology – OncoRay , Chairman of the Company) and Dr. Denis Corbeil ( biotechnology Center Dresden – Biotec) that the intensive preparation of the Congress has been worthwhile. Prof. Dr. Kunz- Schughart : ” The interdisciplinary scientific program with numerous high-profile international experts and pleasing environment were key to the success of the event”.

Last years conference succeeded in building a bridge between multiple cytometric and cell analytical methodologies. It was an excellent opportunity to discuss new technological developments in the context of the many challlenges in biomedical research, that have to be addressed. A special highlight was the presentation by Prof. Dr. David Harris , Arizona , United States , founder of the world’s first cord blood bank on the topic ” The Future of Cord Blood Stem Cell Banking: Regenerative Medicine & Cellular Therapies ” .

Without the proactive participation of all working group members and the support of sponsors and funding agencies in the event of this high quality would not have been possible. For this we are particularly grateful to the organizers . ” At this point in particular, the German Research Foundation ( DFG) and the International Society for Advancement of Cytometry ( ISAC ) should be mentioned.

Plasmonic W..
Start date: October 20
10:00 am 11:30 am

Description: 

Wir sind ein Team von Masterstudenten am BIOTEC der TU Dresden, die am diesjährigen Biomolekularen Design Wettbewerb der Harvard Universität (Boston) teilnehmen. Unser Projekt bezieht sich auf eine futuristische Idee aus dem Bereich der Nanoelektronik. Hierbei wird analog zu Glasfasern Licht entlang von Goldnanopartikeln auf einer DNA Struktur geleitet. Ziel ist es, damit optische Nanoschaltkreise zu konstruieren, die Signale mit annähernder Lichtgeschwindigkeit verarbeiten. Die DNA wird hierfür in einem völlig neuen Kontext, nicht als Erbsubstanz, sondern als Gerüstmaterial verwendet. Mit unserem anschaulichen Lichtmodell würden wir Sie gerne in die Welt der Nanokonstruktion mit Biomolekülen und ihre faszinierenden Möglichkeiten begleiten.

Diabetes
Start date: October 20
04:00 pm 06:00 pm

Description: 

Schedule

4:00-4:05 pm: Introduction

4:05-4:40 pm: Andreas Birkenfeld (MK3/PLID) - "INDY in the regulation of metabolic control and lifespan"

4:40-5:15 pm: Ünal Coskun (PLID) - "Lipid-Protein interactions in cell signaling"

5:15-5:50 pm: Anthony Gavalas (PLID) - "A novel signal for the survival and specification of pancreas progenitors"

5:50 pm onwards: Discussion and social get-together

Join the discussion afterwards, incl. drinks and snacks.

Cells as sm..
Start date: October 24
11:00 am 12:00 pm

Description: 

Please contact the host (jochen.guck(at)biotec.tu-dresden.de) if you would like to talk to the speaker.

Everybody is welcome!

------------------------------------------------------------------------

Abstract:
Living cells are capable of processing a variety of mechanical signals encoded within their microenvironment, which can in turn act through the cellular structural machinery to regulate many fundamental behaviors. In this sense, cells may be regarded as "smart materials” that dynamically and locally modulate their physical properties in response to environmental stimuli.  Here we discuss our recent efforts to dissect, control, and mimic these phenomena. First, we have used laser nanosurgery to spatially map the nanomechanical properties of actomyosin stress fibers. We have combined this approach with advanced molecular imaging tools (FRAP, FRET) to relate intracellular tensile forces to the conformational activation of mechanosensory proteins at the cell-microenvironment interface and the activities of specific myosin activators and isoforms. Second, we have used the tools of synthetic biology to precisely control the expression and activation of mechanoregulatory proteins in single cells using multiple mutually orthogonal inducer/repressor systems. This capability has enabled us to quantitatively elucidate relationships between signal activation and phenotype and to deconstruct complex signaling networks. By combining these genetic approaches with advanced culture paradigms and in vivo models, we have been able to explore how mechanobiological signals may help drive stem cell differentiation and tumor invasion in the central nervous system.  We are now beginning to close the loop by engineering proteins that mimic the stimulus-responsive features of cellular structural networks and may serve as smart, genetically-encoded mechanochemical building blocks.

Publications:
A. J. Keung*, E. M. de Juan-Pardo*, D. V. Schaffer, and S. Kumar (2011).  Rho GTPases Mediate the Mechanosensitive Lineage Commitment of Neural Stem Cells.”  Stem Cells 29: 1886-1897 [*equal contribution].

A. Pathak and S. Kumar (2012).  Independent regulation of tumor cell migration by matrix stiffness and confinement.  Proceedings of the National Academy of Sciences (PNAS) 109: 10334-10339. 

C.-W. Chang and S. Kumar (2013).  Vinculin tension distributions of individual stress fibers within cell-matrix adhesions.  Journal of Cell Science 126: 3021-3030. 

Y. Kim and S. Kumar (2014).  CD44-mediated adhesion to hyaluronic acid contributes to mechanosensing and invasive motility.  Molecular Cancer Research (DOI 10.1158/1541-7786.MCR-13-0629).

N. Srinivasan, M. Bhagawati, B. Ananthanarayanan, and S. Kumar (2014).  Stimuli-sensitive intrinsically disordered protein brushes.  Nature Communications 5: 5145.  

Self-assemb..
Start date: October 24
04:00 pm 05:00 pm

Description: 

Every year, teams of international undergraduate students of more than 30 universities worldwide are taking part in a bio-molecular design competition called “BIOMOD”. Each team develops a science project to create new applications in nanometer scale for medicine, technology and others. Last year´s team of the TU Dresden proved to be extraordinary successful and finished in second place at the winning ceremony, held at the Harvard University. This year, we want to represent again TU Dresden and continue last year’s successful results.
We are a team of 12 international master students in the Center of Biotechnological Innovation of TU Dresden supported by Dr. Thorsten-Lars Schmidt and PHD student Fatih Nadi Gür from CFAED (Center for Advancing Electronics Dresden) and we are one of the only teams from European universities which take the chance of participating in this unique competition.

We are happy to present you the results of our project, we worked on over the summer holidays and we would appreciate your feedback!

Abstract:

Nanoscale devices can be produced from DNA with higher precision and in a more scalable way than with any other available technique. For example, optically active components such as gold nanoparticles can be arranged on DNA scaffolds to create building blocks for future optoelectronic circuitry. Here, we attach functionalized gold nanoparticles (AuNPs) on 6 helix bundle (6HB) DNA origami structures to assemble waveguides. The surface plasmon resonance (SPR) properties of AuNPs allow to transmit electromagnetic waves along the DNA origami templated AuNP chain. Such waveguides are 1000 times smaller than classical optical fibers, and could therefore potentially be integrated into nanometer-sized electrical circuits.

Moreover, we devise an ON/OFF switching system using oligonucleotides as an input signal for this type of DNA origami waveguides. For this, a DNA origami breadboard is used to align two waveguides in close proximity. Single-stranded DNA is used for anchoring the waveguides to the breadboard structure. The waveguides are interconnected via complementary strands. ON/OFF switching of the waveguides is based on reversible hybridization and strand displacement. With this approach, we aim to combine the benefits of parallel DNA computing and superfast photonic circuits.

Datenjongle..
Start date: October 29
10:00 am 11:30 am

Description: 

Moderne Experimente der Molekularbiologie bringen eine Datenflut hervor, die ohne Computer nicht mehr zu beherrschen ist. Hier hilft die Bioinformatik weiter. Sie befasst sich mit der Verarbeitung und Analyse biologischer Daten von Genen und Proteinen über Bilder bis hin zu wissenschaftlichen Texten. Bioinformatiker an der TU Dresden untersuchen Proteine – kleine Helfer, die in jeder Zelle unverzichtbare Arbeit leisten, denn ohne sie wäre kein Leben möglich. Wie die vielen tausend Proteine in einer Zelle zusammenarbeiten oder wie sie im Laufe von Millionen Jahren entstanden sind, ist vielfach noch unbekannt.

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