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October 01 - October 31

10 entries found

10 X Genomi..
Start date: October 01
10:00 am 12:00 pm

Description: 

The seminar will inform you about the single cell sequencing technologies of 10 X Genomics and give you the opportunity to discuss the limitations and challenges of the technology. Furthermore, an overview and first insights into the release of the spatial sequencing technology VISIUM planned for October 2019 will be given. (https://www.10xgenomics.com/spatial-transcriptomics/

Control of ..
Start date: October 01
03:00 pm 04:00 pm

Evolution o..
Start date: October 07
04:00 pm 05:00 pm

Description: 

Abstract
Decades of mouse genetics and chick embryology have identified genes and pathways that are necessary for vertebrate limb development, but loss-of function mutations in the mouse frequently cause proportionate dwarfism that doesn't represent the diverse skeletal proportions observed in nature. In an effort to tap into the naturally occurring genetic “selection experiment” that has been ongoing since the origin of the vertebrate limb, I developed the lesser Egyptian jerboa, Jaculus jaculus, as a new experimental system. The jerboa is closely related to the laboratory mouse, enabling direct comparison to an established model system with very similar genomic architecture and developmental staging, and yet extremely divergent in terms of its limb morphology. This desert-adapted bipedal rodent has extraordinarily long hindlimbs with three toes on its disproportionately large feet and normally proportioned forelimbs with five fingers.  My laboratory takes a hierarchical approach to understand  this evolutionary transformation at the level of tissue architecture and cell behaviors to associated genes and the cis-regulatory modules that allowed the hindlimb to evolve independent of the forelimb.


5 most important publications

Grunwald HA*, Gantz VM*, Poplawski G*, Xu XS, Bier E, Cooper KL^ (2019) Super-Mendelian inheritance mediated by CRISPR/Cas9 in the female mouse germline. Nature. 566(7742): 105-109.

Moore TY, Organ CL, Edwards SV, Biewener AA, Tabin CJ, Jenkins FA Jr, Cooper KL. (2015) Multiple phylogenetically distinct events shaped the evolution of limb skeletal morphologies associated with bipedalism in the jerboas. Current Biology. 25(21): 2785-2794.

Cooper KL*^, Sears KE*^, Uygur A*, Maier J, Baczkowski KS, Brosnahan M, Antczak D, Skidmore JA, Tabin CJ (2014) Patterning and post-patterning modes of evolutionary digit loss in mammals. Nature. 511(7507): 41-45.

Cooper KL*^, Oh S*, Sung Y, Dasari R, Kirschner MK, Tabin CJ (2013) Multiple phases of chondrocyte enlargement underlie differences in skeletal proportions. Nature. 495(7441): 375-378.

Cooper KL*, Hu J*, ten Berge D, Fernandez-Teran M, Ros M, Tabin CJ (2011) Initiation of proximal-distal patterning of the vertebrate limb by signals and growth. Science. 332(6033): 1083-1086.

Everybody is very welcome!

Multicompon..
Start date: October 10
04:00 pm 05:00 pm

Description: 

Abstract:

Activation or inactivation of a living cell, tissue or animal, either naturally or artificially, initiates small or larger long-term changes within the living system (The biological uncertainty principle). In most cases the researcher intends to learn about the living system without being aware about its own – in many cases -destructive  action. Illumination of a cell is probably one the least destructive action one may undertake. We study natural sensory photoreceptors mostly from green algae with respect to the original function within the algal context but also modify these photoreceptors and employ them in host cells to mani-pulate host processes ideally non-invasively. For a long time the membrane voltage has been the main host parameter and we engineered - supported by spectroscopic and structural information - light-gated channels and light-driven pumps in many direction with respect to color sensing, ion selectivity and kinetics, and converted ion pumps into ion channels or vice versa to understand the principle differences. More recently we focused on second messengers as cAMP and cGMP by employment of fungal photo-activated cyclases. The combi¬nation with cyclic nucleotide gated (cNG) channels generated multi-component optogenetic systems with large amplifications providing ultra high sensitivity in host cells.  We keep in mind Max Plancks concept: “Understanding precedes application” and we begin to learn that “manipulation always causes distraction from natural behavior”. Moreover, large signals do not mean natural responses, it may be just the opposite.

5 most important publications:

1.* Harz, H. and Hegemann, P. (1991) Rhodopsin-regulated calcium currents in Chlamydomonas. Nature 351, 489-491.
2.* Nagel, G., Ollig, D., Fuhrmann, M., Kateriya, S., Musti, A.-M., Bamberg, E., Hegemann, P. (2002) Channelrhodopsin-1, a light-gated proton channel in green algae. Science 296, 2395 - 2398.
3.* Stierl, M., Stumpf, P., Udwari, D., Gueta, R., Hagedorn, R., Losi, A., Gärtner, W., Petereit, L., Efetova, M., Schwarzel, M., Oertner, T.G., Nagel, G., and Hegemann, P. (2011) Light-modulation of cellular cAMP by a small bacterial photoactivated adenylyl cyclase, bPAC, J. Biol. Chem. 286: 1181-1188.  
4.* Wietek, J., Wiegert, J.S., Adeishvili, N., Schneider, F., Watanabe, H., Tsunoda, S.P., Vogt, A., Elstner, M., Oertner, T.G., Hegemann, P. (2014) Conversion of Channelrhodopsin into a light-gated chloride channel. Science 344, 409 – 412.
5. * Scheib, U., Stehfest, K., Gee, C. E., Körschen, H.G., Fudim, R., Oertner, T. G., and Hegemann, P. (2015) The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling. Science Signaling 8, 389 rs8.

Süße Algen,..
Start date: October 16
10:00 am 11:30 am

Mechanisms ..
Start date: October 17
04:00 pm 05:00 pm

Description: 

Abstract
Wound repair and regeneration are fundamental features of animal biology, and the capacity to replace all missing tissues (“whole-body regeneration”) is widely distributed across animal phyla. The genetic pathways that mediate whole-body regeneration are poorly understood, and little is known about how these pathways compare across animal lineages. Functional studies of species in phylogenetically informative positions are needed both to elucidate further the mechanisms of regeneration and to evaluate how these mechanisms have evolved. The goals of my research program are: 1) to identify cellular and genetic mechanisms for whole-body regeneration, and 2) to understand how regeneration has evolved by comparing mechanisms across distantly-related animals. We focus our work on a new model system, the acoel worm, Hofstenia miamia, which regenerates robustly and represents the sister-lineage to all other animals with bilateral symmetry, to address these questions. In this talk, I will discuss how we utilize a diversity of approaches including functional genomics, single-cell RNA-sequencing, and transgenesis to uncover the mechanisms of regeneration in Hofstenia.

Publications
1.    A.R. Gehrke, E. Neverett, Y-J. Luo, A. Brandt, L. Ricci, R.E. Hulett, A. Gompers, J.G. Ruby, D.S. Rokhsar, P.W.Reddien, and M. Srivastava. Acoel genome reveals the regulatory landscape of whole-body regeneration. Science,eaau6173:10.1126/science.aau6173, 2019.
2.    Srivastava, M., K. Mazza-Curll, J.C. van Wolfswinkel, and P.W. Reddien (2014). Whole-body acoel regeneration is controlled by Wnt and Bmp-Admp signaling. Curr. Biol. 24(10): 1107-13.
3.    Raz, A., M. Srivastava, R. Salvenmoser, and P. W. Reddien. Acoel regeneration mechanisms indicate an ancient role for muscle in regenerative patterning. 10.1038/s41467-017-01148-5. Nat. Comm., 2017
4.    Srivastava, M., O. Simakov, J. Chapman, B. Fahey, M.E. Gauthier, T. Mitros, G.S. Richards, C. Conaco, M. Dacre,U. Hellsten, C. Larroux, N.H. Putnam, M. Stanke, M. Adamska, A. Darling, S.M. Degnan, T.H. Oakley, D.C. Plachetzki, Y. Zhai, M. Adamski, A. Calcino, S.F. Cummins, D.M. Goodstein, C. Harris, D.J. Jackson, S.P. Leys, S. Shu, B.J.Woodcroft, M. Vervoort, K.S. Kosik, G. Manning, B.M. Degnan, and D.S. Rokhsar (2010). The Amphimedon queenslandica genome and the evolution of animal complexity. Nature, 466(7307): 720–726.
5.    Srivastava, M., E. Begovic, J. Chapman, N. H. Putnam, U. Hellsten, T. Kawashima, A. Kuo, T. Mitros, M.L. Carpenter, A.Y. Signorovitch, M.A. Moreno, K. Kamm, J. Grimwood, J. Schmutz, H. Shapiro, I. V. Grigoriev, L.W. Buss, B. Schierwater, S. Dellaporta, and D. Rokhsar (2008). The Trichoplax genome and the nature of placozoans. Nature, 454(7207): 955–960.

Piconewton-..
Start date: October 24
04:00 pm 05:00 pm

Description: 

Abstract
To investigate the molecular mechanisms underlying cell adhesion mechanics, we developed a set of single-molecule‒calibrated biosensors that are sensitive to physiologically relevant forces in the low piconewton range. These biosensors are genetically encoded and can be utilized to determine molecular forces acting across individual molecules in cells. In my seminar, I will describe how their application to the focal adhesion protein talin and the desmosomal molecule desmoplakin revealed fundamental differences in how distinct adhesion molecules modulate intracellular force transduction.

5 most important publications
1. Price AJ, Cost AL, Ungewiβ H, Waschke J, Dunn AR, and Grashoff C. 2018. Nat Commun 11; 9:5284.
2. Ringer, P. Weißl, A., Cost, A.L., Freikamp, A., Sabass, B., Mehlich, A., Tramier, M., Rief, M. and Grashoff, C. 2017. Nat Methods 14: 1090-1096.
3. Austen KA, Ringer P, Mehlich A, Chrostek-Grashoff A, Kluger C, Klingner C, Sabass B, Zent R, Rief M, and Grashoff C. 2015. Nat Cell Biol 17: 1597-1606.
4. Hoffman BD, Grashoff C, and Schwartz MA. 2011. Nature 475(7356): 316-323.
5. Grashoff C, Hoffman BD, Brenner MD, Zhou R, Parsons M, Yang MT, McLean MA, Sligar SG, Chen CS, Ha T, and Schwartz MA. 2010. Nature 466(7303): 263-266.

Everybody is very welcome!

Technology ..
Start date: October 28
04:00 pm 06:00 pm

Description: 

CRTD Symposium
Technology for Organoids

4:00 - 4:05 Introduction by Carsten Werner

4:05 - 4:25 Byung Ho Lee, Max Planck Institute of Cell Biology and Genetics Dresden
The Influence of Mechanical Cues on Pancreatic Organoid Branching

4:25 - 4:45 Valentina Magno, Leibniz Institute of Polymer Research Dresden
Hydrogels to Modulate the Differentiation of iPSC-derived Kidney Organoids

4:45 - 5:05 Andrea Meinhardt, Leibniz Institute of Polymer Research Dresden
Growth Factor Modulation by Hydrogels to Guide Neuroepithelial Cyst Differentiation

5:05 - 5:35 Daniel Stange, University Hospital Carl Gustav Carus, TU Dresden
Modeling Gastric Cancer using Organoids

5:35 - 6:00 Discussion and social get-together

Post-transc..
Start date: October 29
03:00 pm 04:00 pm

Wie alt ist..
Start date: October 30
10:00 am 11:00 am

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