Examplepictures of DNA-Structures



Cells as smart materials: Quantitatively dissecting and rebuilding mechanibiological units

BIOTEC Green Seminar

Date:24.10.2014, 11:00 - 12:00
Speaker: Prof. Sanjay Kumar, University of California, Berkeley Department of Bioenineering
Location: CRTD, Auditorium left
Host: Jochen Guck

Please contact the host (jochen.guck(at)biotec.tu-dresden.de) if you would like to talk to the speaker.

Everybody is welcome!


Living cells are capable of processing a variety of mechanical signals encoded within their microenvironment, which can in turn act through the cellular structural machinery to regulate many fundamental behaviors. In this sense, cells may be regarded as "smart materials” that dynamically and locally modulate their physical properties in response to environmental stimuli.  Here we discuss our recent efforts to dissect, control, and mimic these phenomena. First, we have used laser nanosurgery to spatially map the nanomechanical properties of actomyosin stress fibers. We have combined this approach with advanced molecular imaging tools (FRAP, FRET) to relate intracellular tensile forces to the conformational activation of mechanosensory proteins at the cell-microenvironment interface and the activities of specific myosin activators and isoforms. Second, we have used the tools of synthetic biology to precisely control the expression and activation of mechanoregulatory proteins in single cells using multiple mutually orthogonal inducer/repressor systems. This capability has enabled us to quantitatively elucidate relationships between signal activation and phenotype and to deconstruct complex signaling networks. By combining these genetic approaches with advanced culture paradigms and in vivo models, we have been able to explore how mechanobiological signals may help drive stem cell differentiation and tumor invasion in the central nervous system.  We are now beginning to close the loop by engineering proteins that mimic the stimulus-responsive features of cellular structural networks and may serve as smart, genetically-encoded mechanochemical building blocks.

A. J. Keung*, E. M. de Juan-Pardo*, D. V. Schaffer, and S. Kumar (2011).  Rho GTPases Mediate the Mechanosensitive Lineage Commitment of Neural Stem Cells.”  Stem Cells 29: 1886-1897 [*equal contribution].

A. Pathak and S. Kumar (2012).  Independent regulation of tumor cell migration by matrix stiffness and confinement.  Proceedings of the National Academy of Sciences (PNAS) 109: 10334-10339. 

C.-W. Chang and S. Kumar (2013).  Vinculin tension distributions of individual stress fibers within cell-matrix adhesions.  Journal of Cell Science 126: 3021-3030. 

Y. Kim and S. Kumar (2014).  CD44-mediated adhesion to hyaluronic acid contributes to mechanosensing and invasive motility.  Molecular Cancer Research (DOI 10.1158/1541-7786.MCR-13-0629).

N. Srinivasan, M. Bhagawati, B. Ananthanarayanan, and S. Kumar (2014).  Stimuli-sensitive intrinsically disordered protein brushes.  Nature Communications 5: 5145.  

Self-assembled Plasmonic Waveguides and Switches for DNA Computing and Nanophotonic Circuits

Extraordinary Friday Seminar

Date:24.10.2014, 16:00 - 17:00
Speaker: TU Dresden BIOMOD 2014 Team - Dresden DNAmic
Location: CRTD, Auditorium left

Every year, teams of international undergraduate students of more than 30 universities worldwide are taking part in a bio-molecular design competition called “BIOMOD”. Each team develops a science project to create new applications in nanometer scale for medicine, technology and others. Last year´s team of the TU Dresden proved to be extraordinary successful and finished in second place at the winning ceremony, held at the Harvard University. This year, we want to represent again TU Dresden and continue last year’s successful results.
We are a team of 12 international master students in the Center of Biotechnological Innovation of TU Dresden supported by Dr. Thorsten-Lars Schmidt and PHD student Fatih Nadi Gür from CFAED (Center for Advancing Electronics Dresden) and we are one of the only teams from European universities which take the chance of participating in this unique competition.

We are happy to present you the results of our project, we worked on over the summer holidays and we would appreciate your feedback!


Nanoscale devices can be produced from DNA with higher precision and in a more scalable way than with any other available technique. For example, optically active components such as gold nanoparticles can be arranged on DNA scaffolds to create building blocks for future optoelectronic circuitry. Here, we attach functionalized gold nanoparticles (AuNPs) on 6 helix bundle (6HB) DNA origami structures to assemble waveguides. The surface plasmon resonance (SPR) properties of AuNPs allow to transmit electromagnetic waves along the DNA origami templated AuNP chain. Such waveguides are 1000 times smaller than classical optical fibers, and could therefore potentially be integrated into nanometer-sized electrical circuits.

Moreover, we devise an ON/OFF switching system using oligonucleotides as an input signal for this type of DNA origami waveguides. For this, a DNA origami breadboard is used to align two waveguides in close proximity. Single-stranded DNA is used for anchoring the waveguides to the breadboard structure. The waveguides are interconnected via complementary strands. ON/OFF switching of the waveguides is based on reversible hybridization and strand displacement. With this approach, we aim to combine the benefits of parallel DNA computing and superfast photonic circuits.

Expression and role of tristetraprolin, a RNA binding protein, in mammary

TU DD Seminar

Date:28.10.2014, 11:00 - 12:00
Speaker: Maria Victoria Goddio, University of Buenos Aires, College of Chemical and Natural Sciences, Argentina
Location: CRTD, Seminar Room 2
Host: Christian Eckmann

Datenjongleure: Bioinformatiker untersuchen Proteine in 3D

Dresden Seniors Academy

Date:29.10.2014, 10:00 - 11:30
Speaker: Janine Roy, BIOTEC
Location: Auditorium
Host: Birte Urban

Moderne Experimente der Molekularbiologie bringen eine Datenflut hervor, die ohne Computer nicht mehr zu beherrschen ist. Hier hilft die Bioinformatik weiter. Sie befasst sich mit der Verarbeitung und Analyse biologischer Daten von Genen und Proteinen über Bilder bis hin zu wissenschaftlichen Texten. Bioinformatiker an der TU Dresden untersuchen Proteine – kleine Helfer, die in jeder Zelle unverzichtbare Arbeit leisten, denn ohne sie wäre kein Leben möglich. Wie die vielen tausend Proteine in einer Zelle zusammenarbeiten oder wie sie im Laufe von Millionen Jahren entstanden sind, ist vielfach noch unbekannt.

Immunological control of hepatic and intestinal homeostasis

CRTD Seminar

Date:10.11.2014, 16:00 - 17:00
Speaker: Prof. Dr. Sebastian Zeissig, Department of Internal Medicine I, University Medical Center Schleswig Holstein Kiel, Christian-Albrechts-University Kiel
Location: CRTD Auditorium left
Host: Elly Tanaka / Jochen Hampe

If you want to talk to the speaker, please contact Andrea Meinhardt (Andrea.Meinhardt@crt-dresden.de). Everybody is welcome! Abstract

My laboratory is interested in the mechanisms which govern homeostasis and prevent inflammation in host organs such as the liver and the intestine. Of particular interest to our studies is the role of parenchymal cells as non-professional antigen-presenting cells in the regulation of innate and adaptive immunity. As such, we could demonstrate that hepatocytes and intestinal epithelial cells can present lipid antigens via the non-classical MHC class I molecule CD1d to natural killer T cells thus providing the basis for protection from infectious hepatitis as well as intestinal inflammation (Zeissig et al., Nat. Med. 2012; Olszak et al., Nature 2014). Furthermore, this work revealed an essential role of the commensal microbiota and microbiota-derived lipid antigens in the control of homeostasis at mucosal surfaces and demonstrated that primary defects in lipid antigen presentation are associated with immunodeficiency in humans (An et al., Cell 2014; Olszak et al., Science 2012; Zeissig et al., J. Clin. Invest.; Zeissig et al., Nat. Immunol. 2014). Current work in the laboratory is focused on the identification of lipid antigens which link metabolism and immunity in the liver and the intestine. Further, we are investigating the role of inflammation-associated pathways in the intestinal epithelium and their contribution to microbiota-dependent intestinal stem cell function and the control of inflammation, regeneration, and carcinogenesis.



An D, Oh SF, Olszak T, Neves JF, Erturk-Hasdemir D, Lu X, Zeissig S, Blumberg RS, Kasper DL. Sphingolipids from a symbiotic microbe regulate homeostasis of host intestinal natural killer T cells. Cell. 2014 Jan 16;156(1-2): 123-33.

Olszak T, Neves JF, Dowds CM, Baker K, Glickman J, Davidson NO, Lin CS, Jobin C, Brand S, Sotlar K, Wada K, Katayama K, Nakajima A, Mizuguchi H, Kawasaki K, Nagata K, Müller W, Snapper SB, Schreiber S, Kaser A, Zeissig S*, Blumberg RS*.  Protective mucosal immunity mediated by epithelial CD1d and IL-10. Nature. 2014 May 22;509(7501):497-502.  (*co-senior authors)

Olszak T, An D, Zeissig S, Vera MP, Richter J, Franke A, Glickman JN, Siebert R, Baron RM, Kasper DL, Blumberg RS. Microbial exposure during early life has persistent effects on tissue-associated iNKT cells and their function. Science. 2012 Apr 27;336(6080):489-93.

Zeissig S, Dougan SK, Barral D, Junker Y, Chen Z, Kaser A, Ho M, Mandel H, McIntyre A, Kennedy SM, Painter, GF, Veerapen N, Besra GS, Cerundolo V, Yue S, Beladi S, Behar SM, Chen X, Gumpertz JE, Breckpot K, Raper A, Baer A, Exley MA, Hegele RA, Cuchel M, Rader DJ, Davidson NO, Blumberg RS, Primary deficiency of microsomal triglyceride transfer protein in human abetalipoproteinemia is associated with loss of CD1 function. J Clin Invest. 2010 Aug 2;120(8):2889-99.

Zeissig S, Blumberg RS. Life at the beginning: perturbation of the microbiota by antibiotics in early life and its role in health and disease. Nat. Immunol. 2014 Mar 19; 15(4): 307-10.

Zeissig S, Murata M, Sweet L, Publicover J, Hu Z, Kaser A, Bosse E, Iqbal J, Hussain MM, Balschun K, Röcken C, Arlt A, Günther R, Hampe J, Schreiber S, Baron JL, Moody DB, Liang JT, Blumberg RS. Hepatitis B virus-induced alterations in hepatocyte CD1d lipid antigens activate natural killer T cells and contribute to protective immunity. Nat. Med. 2012. Jul;18(7):1060-8. 

Neue Therapien für alte Knochen

Dresden Seniors Academy

Date:12.11.2014, 10:00 - 11:30
Speaker: Lorenz Hofbauer, University Hospital Dresden
Location: Auditorium left
Host: Birte Urban

Osteoporose ist eine Knochenkrankheit, die 40% aller Frauen, aber auch jeden  5. Mann trifft. Deutschlandweit leiden mehr als sieben Millionen Menschen an dieser Krankheit - Tendenz steigend. Die Knochen der Osteoporose-Patienten sind weniger dicht und instabiler, wodurch sie leichter brechen können. Vor allem ältere Mitbürger leiden zusätzlich an häufigen Stürzen als Folge der nachlassenden Muskelkraft, eines eingeschränkten Gleichgewichtssinns oder Sehvermögens. Knochenbrüche der Unter- und Oberarmknochen oder der Hüfte können die Folge sein.
In diesem Vortrag stellt Ihnen Professor Lorenz Hofbauer, Bereichsleiter für Endokrinologie, Diabetes und Knochenerkrankungen an der Medizinischen Klinik III des Universitätsklinikum Carl-Gustav-Carus Dresden, sowohl neue Behandlungsmöglichkeiten für Osteoporose als auch vorbeugende Maßnahmen  vor.

Der Fisch kann es. Warum ich nicht?

Dresden Seniors Academy

Date:14.01.2015, 10:00 - 11:30
Speaker: Michell Reimer, CRTD
Location: Seminar Room 3, 3rd Floor
Host: Birte Urban

Verletzungen des zentralen Nervensystems führen bei uns Menschen zu irreparablen funktionalen Beeinträchtigungen. Im Gegensatz hierzu besitzt der Modellorganismus Zebrafisch - auch ein Wirbeltier - die Fähigkeit, ein beschädigtes zentrales Nervensystem zu reparieren. Hierbei werden neue Neurone geboren, in das noch bestehende neuronale Netzwerk integriert  und Verbindungen zwischen Nervenzellen geknüpft. Eine vollständige funktionelle Erholung bildet beim Zebrafisch den Abschluss dieses komplexen Regenerationsprozesses. Wie der Fisch dieses Kunststück meistert, und ob dies langfristig eventuell auf den Menschen übertragen werden kann, erfahren Sie in diesem Vortrag.

Warum Studenten die schwimmende Maus spielen - Gedanken zur translationalen Forschung

Dresden Seniors Academy

Date:04.02.2015, 10:00 - 11:30
Speaker: Alexander Garthe, CRTD + DZNE
Location: Seminar Room 3, 3rd Floor
Host: Birte Urban

Ziel der Grundlagenforschung ist das Verständnis biologischer Mechanismen und die Identifizierung potenzieller Ansatzpunkte für die Entwicklung neuartiger Therapie- oder Präventionsstrategien. Hierzu wird in der Regel an Zellkulturen, isolierten Gewebeteilen oder Modellorganismen wie Fischen, Mäusen und Ratten geforscht. Dies wirft früher oder später die Frage auf, wie zuverlässig sich komplexe biologische Vorgänge im menschlichen Organismus korrekt mit solchen Modellen abbilden lassen. Besonders kritisch wird diese Frage bei der Erforschung von Hirnfunktionen und deren (oftmals mit fortgeschrittenem Alter assoziierten) Veränderung. Können Mäuse denken? Sind wir Menschen immer und überall schlauer? Und welche Rolle spielt unsere üblicherweise viel höhere Lebensspanne?

Der Vortrag erläutert diese und andere Aspekte auf anschauliche Weise und  stellt einen gemeinsam mit dem Institut für Sportmedizin und –biologie der TU Chemnitz entwickelten Lerntest vor, der uns Menschen einige interessante Momente mit den Augen einer Maus nachempfinden lässt.

Mit Vollgas durchs Genom: Hochdurchsatzsequenzierung zur Entschlüsselung des Erbgutes

Dresden Seniors Academy

Date:11.02.2015, 10:00 - 11:30
Speaker: Andreas Dahl, SFB 655
Location: Seminar Room 3, 3rd Floor
Host: Birte Urban

Die Sequenzierung des ersten menschlichen Genoms hat zehn Jahre gedauert und rund 2 Milliarden Dollar gekostet. Durch die technologischen Fortschritte in der Molekularbiologie in den letzten Jahren wurde die Geschwindigkeit, mit der die DNA-Sequenz entschlüsselt werden kann, so beschleunigt, dass dies aktuell in wenigen Tagen zu einem Bruchteil der Kosten des Humanen Genomprojektes möglich ist. Erfahren Sie mehr über die technologischen Konzepte und Tricks und deren Einfluss auf die aktuelle Forschung in Biologie und Medizin.

Double Conference on Program of Early Mammalian Development and Systems Biology of Stem Cells

Date:08.03.2015, 16:00 - 12.03.2015, 12:00
Location: Oberstdorf-Haus, Oberstdorf, Germany
Host: Francis Stewart, Andreas Beyer, Edith Heard, Austin Smith

Program of Early Mammalian Development (08-11 March) and Systems Biology of Stem Cells (10-12 March) are the two overlapping conferences with two joint sessions organized by the EU-funded SyBoSS project. A large number of excellent speakers has followed our invitation to winterly Oberstdorf, the most southern village in Germany. Among them will be:
Adrian Bird, Déborah Bourc'his, Anne Ferguson-Smith, Kat Hadjantonakis, Petra Hajkova, Hitoshi Niwa, Mitinori Saitou, Jörn Walter, Raja Jothi, Nevan Krogran, Bo Torben Porse, Mike Rudnicki, Timm Schroeder and many more.

For more information see here: http://syboss.eu/oberstdorf

More speakers will be selected from abstracts.

Here you can download the poster

Register now!


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