Primary Research Publications
Nedelkova, M., Maresca, M., Fu, J., Rostovskaya, M., Chenna, R., Thiede, C., Anastassiadis, K., Sarov, M. and Stewart, A. F. (2011). "Targeted isolation of cloned genomic regions by recombineering for haplotype phasing and isogenic targeting." Nucleic Acids Res.
Daniel, K., Lange, J., Hached, K., Fu, J., Anastassiadis, K., Roig, I., Cooke, H. J., Stewart, A. F., Wassmann, K., Jasin, M., Keeney, S. and Toth, A. (2011). "Meiotic homologue alignment and its quality surveillance are controlled by mouse HORMAD1." Nat Cell Biol 13:599-610.
Suckale, J., Wendling, O., Masjkur, J., Jager, M., Munster, C., Anastassiadis, K., Stewart, A. F. and Solimena, M. (2011). "PTBP1 Is Required for Embryonic Development before Gastrulation." PLoS One 6: e16992.
Liu, Y., Suckale, J., Masjkur, J., Magro, M. G., Steffen, A., Anastassiadis, K. and Solimena, M. (2010). "Tamoxifen-Independent Recombination in the RIP-CreER Mouse." PLoS One 5: e13533.
Knopf, F., Schnabel, K., Haase, C., Pfeifer, K., Anastassiadis, K. and Weidinger, G. (2010). "Dually inducible TetON systems for tissue-specific conditional gene expression in zebrafish." Proc Natl Acad Sci U S A.
Andreu-Vieyra, C. V., Chen, R., Agno, J. E., Glaser, S., Anastassiadis, K., Stewart, A. F. and Matzuk, M. M. (2010). "MLL2 Is Required in Oocytes for Bulk Histone 3 Lysine 4 Trimethylation and Transcriptional Silencing." PLoS Biol 8.
Kranz, A., Fu, J., Duerschke, K., Weidlich, S., Naumann, R., Stewart, A. F. and Anastassiadis, K. (2010). "An improved Flp deleter mouse in C57Bl/6 based on Flpo recombinase." Genesis.
Anastassiadis, K., Rostovskaya, M., Lubitz, S., Weidlich, S. and Stewart, A. F. (2010). "Precise conditional immortalization of mouse cells using tetracycline-regulated SV40 large T-antigen." Genesis
Anastassiadis, K., Fu, J., Patsch, C., Hu, S., Weidlich, S., Duerschke, K., Buchholz, F., Edenhofer, F. and Stewart, A. F. (2009). "Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice." Dis Model Mech 2: 508-15.
Cambridge, S. B., Geissler, D., Calegari, F., Anastassiadis, K., Hasan, M. T., Stewart, A. F., Huttner, W. B., Hagen, V. and Bonhoeffer, T. (2009). "Doxycycline-dependent photoactivated gene expression in eukaryotic systems." Nat Methods 6: 527-31.
Ding, L., Paszkowski-Rogacz, M., Nitzsche, A., Slabicki, M. M., Heninger, A. K., de Vries, I., Kittler, R., Junqueira, M., Shevchenko, A., Schulz, H., Hubner, N., Doss, M. X., Sachinidis, A., Hescheler, J., Iacone, R., Anastassiadis, K., Stewart, A. F., Pisabarro, M. T., Caldarelli, A., Poser, I., Theis, M. and Buchholz, F. (2009). "A genome-scale RNAi screen for Oct4 modulators defines a role of the Paf1 complex for embryonic stem cell identity." Cell Stem Cell 4: 403-15.
Glaser, S., Lubitz, S., Loveland, K. L., Ohbo, K., Robb, L., Schwenk, F., Seibler, J., Roellig, D., Kranz, A., Anastassiadis, K. and Stewart, A. F. (2009). "The histone 3 lysine 4 methyltransferase, Mll2, is only required briefly in development and spermatogenesis." Epigenetics Chromatin 2: 5.
Schulz, H., Kolde, R., Adler, P., Aksoy, I., Anastassiadis, K., Bader, M., Billon, N., Boeuf, H., Bourillot, P. Y., Buchholz, F., Dani, C., Doss, M. X., Forrester, L., Gitton, M., Henrique, D., Hescheler, J., Himmelbauer, H., Hubner, N., Karantzali, E., Kretsovali, A., Lubitz, S., Pradier, L., Rai, M., Reimand, J., Rolletschek, A., Sachinidis, A., Savatier, P., Stewart, F., Storm, M. P., Trouillas, M., Vilo, J., Welham, M. J., Winkler, J., Wobus, A. M. and Hatzopoulos, A. K. (2009). "The FunGenES database: a genomics resource for mouse embryonic stem cell differentiation." PLoS One 4: e6804.
Vintersten, K., Testa, G., Naumann, R., Anastassiadis, K. and Stewart, A. F. (2008). "Bacterial artificial chromosome transgenesis through pronuclear injection of fertilized mouse oocytes." Methods Mol Biol 415: 83-100.
Blaschke, R. J., Hahurij, N. D., Kuijper, S., Just, S., Wisse, L. J., Deissler, K., Maxelon, T., Anastassiadis, K., Spitzer, J., Hardt, S. E., Scholer, H., Feitsma, H., Rottbauer, W., Blum, M., Meijlink, F., Rappold, G. and Gittenberger-de Groot, A. C. (2007). "Targeted mutation reveals essential functions of the homeodomain transcription factor Shox2 in sinoatrial and pacemaking development." Circulation 115: 1830-8.
Lubitz, S., Glaser, S., Schaft, J., Stewart, A. F. and Anastassiadis, K. (2007). "Increased apoptosis and skewed differentiation in mouse embryonic stem cells lacking the histone methyltransferase Mll2." Mol Biol Cell 18: 2356-66.
Glaser, S., Schaft, J., Lubitz, S., Vintersten, K., van der Hoeven, F., Tufteland, K. R., Aasland, R., Anastassiadis, K., Ang, S. L. and Stewart, A. F. (2006). "Multiple epigenetic maintenance factors implicated by the loss of Mll2 in mouse development." Development 133: 1423-32.
Schnutgen, F., Stewart, A. F., von Melchner, H. and Anastassiadis, K. (2006). "Engineering embryonic stem cells with recombinase systems." Methods Enzymol 420: 100-36.
Testa, G., Schaft, J., van der Hoeven, F., Glaser, S., Anastassiadis, K., Zhang, Y., Hermann, T., Stremmel, W. and Stewart, A. F. (2004). "A reliable lacZ expression reporter cassette for multipurpose, knockout-first alleles." Genesis 38: 151-8.
Anastassiadis, K., Kim, J., Daigle, N., Sprengel, R., Scholer, H. R. and Stewart, A. F. (2002). "A predictable ligand regulated expression strategy for stably integrated transgenes in mammalian cells in culture." Gene 298: 159-72.
Kirchhof, N., Carnwath, J. W., Lemme, E., Anastassiadis, K., Scholer, H. and Niemann, H. (2000). "Expression pattern of Oct-4 in preimplantation embryos of different species." Biol Reprod 63: 1698-705.
Voss, A. K., Thomas, T., Petrou, P., Anastassiadis, K., Scholer, H. and Gruss, P. (2000). "Taube nuss is a novel gene essential for the survival of pluripotent cells of early mouse embryos." Development 127: 5449-61.
Botquin, V., Hess, H., Fuhrmann, G., Anastassiadis, C., Gross, M. K., Vriend, G. and Scholer, H. R. (1998). "New POU dimer configuration mediates antagonistic control of an osteopontin preimplantation enhancer by Oct-4 and Sox-2." Genes Dev 12: 2073-90.
De Felice, M., Ovitt, C., Biffali, E., Rodriguez-Mallon, A., Arra, C., Anastassiadis, K., Macchia, P. E., Mattei, M. G., Mariano, A., Scholer, H., Macchia, V. and Di Lauro, R. (1998). "A mouse model for hereditary thyroid dysgenesis and cleft palate." Nat Genet 19: 395-8.
Nichols, J., Zevnik, B., Anastassiadis, K., Niwa, H., Klewe-Nebenius, D., Chambers, I., Scholer, H. and Smith, A. (1998). "Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4." Cell 95: 379-91.
Anastassiadis, C., Rottmann, O., Hörmansdorfer, S. and Pirchner, F. (1997). "Expression of the hepatitis B surface antigen (HBsAg) under the control of mMT-1 promoter in transgenic mice can be induced by zinc sulphate, dexamethasone and lipopolysaccharide." J. Anim. Breed. Genet. 114: 225-230.
Anastassiadis, C., Leyhe, B., Olsaker, I., Friedl, R., Rottmann, O., Hiendleder, S. and Erhardt, G. (1996). "Three polymorphic microsatellites for bovine chromosomes 7, 12 and 19." Anim Genet 27: 125-6.
Fuchs, H., Anastassiadis, C., Schlee, P., Stein, H. and Rottmann, O. (1996). "Detection of a G/T base substitution in two bleak (Alburnus alburnus) populations by RAPD." J. Appl. Ichtyol. 12: 95-97.
Kuhn, R., Anastassiadis, C. and Pirchner, F. (1996). "Transfer of bovine microsatellites to the cervine (Cervus elaphus)." Anim Genet 27: 199-201.
Anastassiadis, K., Rottmann, O., Weniger, J. H. and Pirchner, F. (1995). "Integration, Transmission und Expression von Transgenen in Mäuselinien." J. Anim. Breed. Genet. 112: 213-223.
Anastassiadis, K. and Rottmann, O. (1994). "Embryotransfer bei der Maus - Einfluß von Genotyp und anderen Faktoren." Archiv für Tierzucht 37: 461-469.
Reviews
Hofemeister, H., Ciotta, G., Fu, J., Seibert, P. M., Schulz, A., Maresca, M., Sarov, M., Anastassiadis, K. and Stewart, A. F. (2010). "Recombineering, transfection, Western, IP and ChIP methods for protein tagging via gene targeting or BAC transgenesis." Methods.
Ciotta, G., Hofemeister, H., Maresca, M., Fu, J., Sarov, M., Anastassiadis, K. and Stewart, A. F. (2010). "Recombineering BAC transgenes for protein tagging." Methods.
Anastassiadis, K., Glaser, S., Kranz, A., Bernhardt, K. and Stewart, A. F. (2010). "A Practical Summary of Site-Specific Recombination, Conditional Mutagenesis, and Tamoxifen Induction of CreERT2." Methods Enzymol 477C: 109-123.
Fu, J., Teucher, M., Anastassiadis, K., Skarnes, W. and Stewart, A. F. (2010). "A Recombineering Pipeline to Make Conditional Targeting Constructs." Methods Enzymol 477C: 125-144.
Glaser, S., Anastassiadis, K. and Stewart, A. F. (2005). "Current issues in mouse genome engineering." Nat Genet 37: 1187-93.
Pesce, M., Anastassiadis, K. and Scholer, H. R. (1999). "Oct-4: lessons of totipotency from embryonic stem cells." Cells Tissues Organs 165: 144-52.
