Examplepictures of DNA-Structures


Protein Engineering in Optogenetics

CMCB external seminar

Datum:14.06.2018, 16:00 - 17:00
Sprecher: Dr. Oliver Griesbeck, Max Planck Institute of Neurobiology, Martinsried
Ort: CRTD, ground floor, auditorium left
Gastgeber: Dr. Veronika Kuscha (CRTD)


Protein-based tools are at the core of many of strategies to study brain function as they endow a high degree of specificity, allow highlighting designated types of neurons and their subcellular structures, and permit implantation deeply into living brains.  Proteins are used as sensors to monitor activity or biochemistry of neurons, to control or block action potential firing of neurons, or to manipulate circuits on longer time scales by pharmaco-genetics. As such, they typically exploit the talents evolved in nature, but they rarely come to application in their native form. Often, many iterative cycles of improvements and adaptations are necessary, domains of different origins may need to be recombined, and often several screening and design strategies may need to be tried and validated to finally obtain a variant that performs highly in a real physiological experiment.  In my presentation I will outline some of the multidisciplinary optimization approaches used in the field to create useful tools for research in cell biology and neuroscience.

Recent Publications:

C. SCHNAITMANN, V. HAIKALA, E. ABRAHAM, V. OBERHAUSER, T. THESTRUP, O. GRIESBECK, D.F. REIFF (2018). Color Processing in the Early Visual System of Drosophila. Cell 172:318–330.

T. THESTRUP, J. LITZLBAUER, I. BARTHOLOMÄUS , M. MUES, L. RUSSO,  H. DANA, Y. KOVALCHUK, Y. LIANG, G. WITTE, T.W. CHEN, S. BECKER, Y. LAUKAT, T. ALLEN, L.C. ROME, A. GEIGER , D.S. KIM, O. GARASCHUK, C. GRIESINGER, O. GRIESBECK (2014). Optimized ratiometric calcium sensors for functional in vivo imaging of neurons and T-lymphocytes. Nature Methods 11:175-82.

Q.-T. NGUYEN, L. F. SCHROEDER, M. MANK , A. MULLER, P. W. TAYLOR, O. GRIESBECK, D. KLEINFELD (2010). An in vivo biosensor for neurotransmitter release and exogenous receptor activity. Nature Neuroscience 13:127-32.

M.MANK, A. FERRAO SANTOS, S. DIRENBERGER, T. MRSIC-FLÖGEL, S. HOFER, V. STEIN, C. LEVELT, D.F. REIFF, T. HENDL, A. BORST, T. BONHOEFFER, M.HÜBENER, O. GRIESBECK (2008). A genetically encoded calcium indicator for chronic in vivo two photon imaging. Nature Methods 5:805-811.

O. GRIESBECK, G.S. BAIRD, R.E. CAMPBELL, D.A. ZACHARIAS, R.Y. TSIEN (2001). Reducing the environmental sensitivity of yellow fluorescent protein: mechanism and application. J. Biol. Chem. 276:29188-94.

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